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PLA2G1B Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420861

MBS420861 is an antibody targeting PLA2G1B. Also known as Goat anti-PLA2G1B Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLA2G1B, Polyclonal Antibody
Full Product Name
Goat anti-PLA2G1B Antibody
Product Gene Name
anti-PLA2G1B antibody
Product Synonym Names
PLA2G1B; phospholipase A2, group IB (pancreas); MGC119834; MGC119835; PLA2; PLA2A; PPLA2; phospholipase A2, group IB; PLA2G1B antibody; phospholipase A2; group IB (pancreas) antibody; MGC119834 antibody; MGC119835 antibody; PLA2 antibody; PLA2A antibody; PPLA2 antibody; phospholipase A2; group IB antibody
Immunogen Sequence
NKAHKNLDTK KYCQS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PLA2G1B peptide (MBS425980) is used for blocking the activity of PLA2G1B antibody (MBS420861)
Molecular Weight
16,360 Da
OMIM
172410
Immunogen
Peptide with sequence NKAHKNLDTKKYCQS, from the C Terminus of the protein sequence according to NP_000919.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PLA2G1B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PLA2G1B antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx.17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx.18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01ug/ml.

Western Blot (WB) of anti-PLA2G1B antibody

(0. 01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PLA2G1B Polyclonal Antibody – Cat# MBS420861

Western Blot (WB) of anti-PLA2G1B antibody

HEK293 overexpressing PLA2G1B (RC216089) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-PLA2G1B Polyclonal Antibody, Western Blot (WB) – Cat# MBS420861

Other Notes

Small volumes of anti-PLA2G1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLA2G1B. It may not necessarily be applicable to this product.

NCBI GI #
4505847
NCBI GeneID
5319
NCBI Accession #
NP_000919.1
UniProt Secondary Accession #
Q3KPI1; B2R4H5
UniProt Related Accession #
P04054
GenBank Nucleotide #
NM_000928.2
NCBI Official Full Name
phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2 group IB
NCBI Official Symbol
PLA2G1B
NCBI Official Synonym Symbols
PLA2; PLA2A; PPLA2
UniProt Protein Name
Phospholipase A2
UniProt Synonym Protein Names
Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
Protein Family
Phospholipase
UniProt Gene Name
PLA2G1B
UniProt Synonym Gene Names
PLA2; PLA2A; PPLA2
UniProt Entry Name
PA21B_HUMAN
NCBI Protein Information
phospholipase A2

NCBI Summary for PLA2G1B

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]
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UniProt Comments for PLA2G1B

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Secreted; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; DNA replication

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; extracellular region; extracellular space; secretory granule

Molecular Function: bile acid binding; calcium ion binding; calcium-dependent phospholipase A2 activity; phospholipase A2 activity; receptor binding

Biological Process: actin filament organization; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of phospholipase A2; antibacterial humoral response; arachidonic acid secretion; cellular response to insulin stimulus; defense response to Gram-positive bacterium; fatty acid biosynthetic process; glucose transport; innate immune response in mucosa; interleukin-8 production; leukotriene biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; neutrophil mediated immunity; phosphatidic acid biosynthetic process; phosphatidylcholine metabolic process; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of immune response; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; signal transduction
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Research Articles on PLA2G1B

1. The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PLA2G1B antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram

Diseases associated with anti-PLA2G1B antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with PLA2G1B and Inflammation
 Weight Gain Antibodies  >3 publications with PLA2G1B and Weight Gain
 Lung Diseases Antibodies  >3 publications with PLA2G1B and Lung Diseases
 Hypertension Antibodies  >2 publications with PLA2G1B and Hypertension
 Atherosclerosis Antibodies  >2 publications with PLA2G1B and Atherosclerosis
 Fatty Liver Antibodies  >2 publications with PLA2G1B and Fatty Liver
 Cardiovascular Diseases Antibodies  >2 publications with PLA2G1B and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with PLA2G1B and Carcinoma, Hepatocellular
 Adenocarcinoma Antibodies  >1 publications with PLA2G1B and Adenocarcinoma
 Hemorrhage Antibodies  >1 publications with PLA2G1B and Hemorrhage

Organs/Tissues associated with anti-PLA2G1B antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >5 publications with PLA2G1B and Pancreas
 Testis Antibodies  >2 publications with PLA2G1B and Testis
 Kidney Antibodies  >1 publications with PLA2G1B and Kidney
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