Goat Anti-ARHGEF5 Polyclonal Antibody – Cat# MBS420902

(0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ARHGEF5 Antibody – Goat Polyclonal

Name: ARHGEF5 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420902
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420902

MBS420902 is an antibody targeting ARHGEF5. Also known as Goat anti-GEF5 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GEF5 (ARHGEF5), Polyclonal Antibody

Full Product Name

Goat anti-GEF5 Antibody

Product Gene Name

anti-ARHGEF5 antibody

Product Synonym Gene Name

GEF5

Product Synonym Names

GEF5; DKFZp686N1969; ARHGEF5; Rho guanine nucleotide exchange factor (GEF) 5; P60; TIM; TIM1; guanine nucleotide regulatory protein TIM; oncogene TIM rho guanine nucleotide exchange factor 5; transforming immortalized mammary oncogene; GEF5 antibody; DKFZp686N1969 antibody; ARHGEF5 antibody; Rho guanine nucleotide exchange factor (GEF) 5 antibody; P60 antibody; TIM antibody; TIM1 antibody; guanine nucleotide regulatory protein TIM antibody; oncogene TIM rho guanine nucleotide exchange factor 5 antibody; transforming immortalized mammary oncogene antibody

Immunogen Sequence

DKEIDQNSQQ EE

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

GEF5 peptide (MBS426638) is used for blocking the activity of GEF5 antibody (MBS420902)

Molecular Weight

60,056 Da

OMIM

600888

Immunogen

Peptide with sequence C-DKEIDQNSQQEE, from the internal region of the protein sequence according to NP_005426.2.

Epitope

Internal region

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-ARHGEF5 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-ARHGEF5 antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3ug/ml.

Other Notes

Small volumes of anti-ARHGEF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF5. It may not necessarily be applicable to this product.

NCBI GI #

50959191

NCBI GeneID

7984

NCBI Accession #

NP_005426.2

UniProt Secondary Accession #

Q6ZML7; A6NNJ2

UniProt Related Accession #

Q12774

GenBank Nucleotide #

NM_005435.3

NCBI Official Full Name

rho guanine nucleotide exchange factor 5

NCBI Official Synonym Full Names

Rho guanine nucleotide exchange factor 5

NCBI Official Symbol

ARHGEF5

NCBI Official Synonym Symbols

P60; TIM; GEF5; TIM1

UniProt Protein Name

Rho guanine nucleotide exchange factor 5

UniProt Synonym Protein Names

Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM

Protein Family

Rho guanine nucleotide exchange factor

UniProt Gene Name

ARHGEF5

UniProt Synonym Gene Names

TIM

UniProt Entry Name

ARHG5_HUMAN

NCBI Protein Information

rho guanine nucleotide exchange factor 5

NCBI Summary for ARHGEF5

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]

UniProt Comments for ARHGEF5

ARHGEF5: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; Motility/polarity/chemotaxis; Oncoprotein; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell junction; cell projection; cytoplasm; nucleoplasm; nucleus; plasma membrane; podosome

Molecular Function: GTP binding; guanyl-nucleotide exchange factor activity; lipid binding; protein binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: actin cytoskeleton organization and biogenesis; myeloid dendritic cell chemotaxis; positive regulation of GTPase activity; positive regulation of JNK activity; positive regulation of stress fiber formation; positive regulation of transcription factor activity; regulation of actin cytoskeleton organization and biogenesis; regulation of cytoskeleton organization and biogenesis; regulation of GTPase activity; regulation of Rho protein signal transduction

Research Articles on ARHGEF5

1. Data indicate that Rho guanine nucleotide exchange factor 5 (ARHGEF5) is expressed in most human lung adenocarcinoma tissues and cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARHGEF5 antibody

Products by Pathway	Pathway Diagram
Regulation Of RhoA Activity Pathway antibodies	Regulation Of RhoA Activity Pathway Diagram

Diseases associated with anti-ARHGEF5 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>2 publications with ARHGEF5 and Breast Neoplasms
Adenocarcinoma Antibodies	>1 publications with ARHGEF5 and Adenocarcinoma
Seizures Antibodies	>1 publications with ARHGEF5 and Seizures
Disease Models, Animal Antibodies	>1 publications with ARHGEF5 and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>1 publications with ARHGEF5 and Cell Transformation, Neoplastic
Inflammation Antibodies	>1 publications with ARHGEF5 and Inflammation

Organs/Tissues associated with anti-ARHGEF5 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with ARHGEF5 and Brain
Lung Antibodies	>2 publications with ARHGEF5 and Lung
Bone Antibodies	>1 publications with ARHGEF5 and Bone
Mouth Antibodies	>1 publications with ARHGEF5 and Mouth
Bone Marrow Antibodies	>1 publications with ARHGEF5 and Bone Marrow
Skin Antibodies	>1 publications with ARHGEF5 and Skin
Kidney Antibodies	>1 publications with ARHGEF5 and Kidney