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GRAP2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420932

MBS420932 is an antibody targeting GRAP2. Also known as Goat anti-GRAP2/GRID/Grf40 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GRAP2/GRID/Grf40, Polyclonal Antibody
Full Product Name
Goat anti-GRAP2/GRID/Grf40 Antibody
Product Gene Name
anti-GRAP2 antibody
Product Synonym Gene Name
GRID; Grf40
Product Synonym Names
GRAP2; GRID; P38; GADS; GRPL; GrbX; Mona; GRB2L; GRBLG; GRAP-2; GRB2-related adaptor protein 2; GRB-2-like protein; SH3-SH2-SH3 adaptor molecule; growth factor receptor-binding protein; GRB2-related protein with insert domain; hematopoietic cell-associate; GRAP2 antibody; GRID antibody; P38 antibody; GADS antibody; GRPL antibody; GrbX antibody; Mona antibody; GRB2L antibody; GRBLG antibody; GRAP-2 antibody; GRB2-related adaptor protein 2 antibody; GRB-2-like protein antibody; SH3-SH2-SH3 adaptor molecule antibody; growth factor receptor-binding protein antibody; GRB2-related protein with insert domain antibody; hematopoietic cell-associated adaptor protein GRPL antibody; growth factor receptor-bound protein 2-related adaptor protein 2 antibody; RP3-370M22.1 antibody; Grf40 antibody; Mona antibody; GRAP2 / GRID / Grf40
Immunogen Sequence
GLFPANYVAP MTR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GRAP2 / GRID / Grf40 peptide (MBS425763) is used for blocking the activity of GRAP2/GRID/Grf40 antibody (MBS420932)
Molecular Weight
24,382 Da
OMIM
604518
Immunogen
Peptide with sequence C-GLFPANYVAPMTR, from the C Terminus of the protein sequence according to NP_004801.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GRAP2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GRAP2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-GRAP2 antibody

HEK293 overexpressing GRAP2 (RC206546) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-GRAP2 Polyclonal Antibody – Cat# MBS420932

Western Blot (WB) of anti-GRAP2 antibody

Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-GRAP2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS420932

Other Notes

Small volumes of anti-GRAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRAP2. It may not necessarily be applicable to this product.

NCBI GI #
4758476
NCBI GeneID
9402
NCBI Accession #
NP_004801.1
UniProt Secondary Accession #
O43726; Q9NRB7; B7Z8I3
UniProt Related Accession #
O75791
GenBank Nucleotide #
NM_004810.3
NCBI Official Full Name
GRB2-related adapter protein 2 isoform 1
NCBI Official Synonym Full Names
GRB2-related adaptor protein 2
NCBI Official Symbol
GRAP2
NCBI Official Synonym Symbols
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
UniProt Protein Name
GRB2-related adapter protein 2
UniProt Synonym Protein Names
Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; P38; Protein GADS; SH3-SH2-SH3 adapter Mona
Protein Family
GRB2-related adapter protein
UniProt Gene Name
GRAP2
UniProt Synonym Gene Names
GADS; GRB2L; GRID; GRB2L; Grf-40
UniProt Entry Name
GRAP2_HUMAN
NCBI Protein Information
GRB2-related adapter protein 2

NCBI Summary for GRAP2

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for GRAP2

GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytoplasm; cytosol; endosome; nucleoplasm; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: cell-cell signaling; positive regulation of signal transduction; Ras protein signal transduction; T cell costimulation; T cell receptor signaling pathway
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Research Articles on GRAP2

1. GADS deficient T cells displayed similar levels of T cell receptor -induced SLP-76 and PLC-gamma1 phosphorylation but exhibited substantial decrease in TCR-induced IL-2 and IFN-gamma release.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GRAP2 antibody

 Reference Product  PubMed Publications
 LAT antibody  >26 publications with GRAP2 and LAT
 CD28 antibody  >7 publications with GRAP2 and CD28
 CBL antibody  >6 publications with GRAP2 and CBL
 SHC1 antibody  >3 publications with GRAP2 and SHC1
 FYB antibody  >3 publications with GRAP2 and FYB
 GAB2 antibody  >2 publications with GRAP2 and GAB2
 SOS1 antibody  >2 publications with GRAP2 and SOS1
 GAB1 antibody  >2 publications with GRAP2 and GAB1

Pathways associated with anti-GRAP2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 FCERI Mediated Ca+2 Mobilization Pathway antibodies  FCERI Mediated Ca+2 Mobilization Pathway Diagram
 FCERI Mediated MAPK Activation Pathway antibodies  FCERI Mediated MAPK Activation Pathway Diagram
 Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies  Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-GRAP2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >3 publications with GRAP2 and Breast Neoplasms
 Disease Models, Animal Antibodies  >3 publications with GRAP2 and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with GRAP2 and Liver Diseases
 Liver Neoplasms Antibodies  >2 publications with GRAP2 and Liver Neoplasms
 Lung Neoplasms Antibodies  >2 publications with GRAP2 and Lung Neoplasms
 Neurologic Manifestations Antibodies  >1 publications with GRAP2 and Neurologic Manifestations
 Male Urogenital Diseases Antibodies  >1 publications with GRAP2 and Male Urogenital Diseases
 Genital Diseases, Female Antibodies  >1 publications with GRAP2 and Genital Diseases, Female
 Inflammation Antibodies  >1 publications with GRAP2 and Inflammation

Organs/Tissues associated with anti-GRAP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >42 publications with GRAP2 and Blood
 Brain Antibodies  >6 publications with GRAP2 and Brain
 Spleen Antibodies  >5 publications with GRAP2 and Spleen
 Thymus Antibodies  >5 publications with GRAP2 and Thymus
 Liver Antibodies  >3 publications with GRAP2 and Liver
 Intestine Antibodies  >2 publications with GRAP2 and Intestine
 Lung Antibodies  >2 publications with GRAP2 and Lung
 Bone Marrow Antibodies  >2 publications with GRAP2 and Bone Marrow
 Pharynx Antibodies  >1 publications with GRAP2 and Pharynx
 Heart Antibodies  >1 publications with GRAP2 and Heart
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