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CD32/FCGR2B (FCGR2B) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420954

MBS420954 is an antibody targeting FCGR2B. Also known as Goat anti-CD32/FCGR2B Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD32/FCGR2B, Polyclonal Antibody
Full Product Name
Goat anti-CD32/FCGR2B Antibody
Product Gene Name
anti-FCGR2B antibody
Product Synonym Gene Name
CD32
Product Synonym Names
FCGR2B; CD32; FCG2; FCGR2; IGFR2; Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIb; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); CD32B; Fc fragment of IgG, low aff; FCGR2B antibody; CD32 antibody; FCG2 antibody; FCGR2 antibody; IGFR2 antibody; Fc fragment of IgG; low affinity IIb; receptor for (CD32) antibody; Fc gamma RIIb antibody; Fc fragment of IgG; low affinity II; receptor for (CD32) antibody; Fc fragment of IgG; low affinity IIb; receptor (CD32) antibody; CD32B antibody; Fc fragment of IgG; low affinity IIb; receptor antibody; CD32 / FCGR2B
Immunogen Sequence
PDALEEPDDQ NRI
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
CD32 / FCGR2B peptide (MBS425840) is used for blocking the activity of CD32/FCGR2B antibody (MBS420954)
Molecular Weight
33,450 Da
OMIM
152700
Immunogen
Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1.
Epitope
C Terminus
Note
This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-FCGR2B antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-FCGR2B antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in Human Placenta. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34. 0kDa according to NP_003992.3;). In transfected HEK293 transiently expressing CD32 a band of approx. 40kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-FCGR2B antibody

(0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-FCGR2B Polyclonal Antibody – Cat# MBS420954

Western Blot (WB) of anti-FCGR2B antibody

HEK293 overexpressing CD32 (RC211982) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-FCGR2B Polyclonal Antibody, Western Blot (WB) – Cat# MBS420954

Immunohistochemistry (IHC) of anti-FCGR2B antibody

(3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-FCGR2B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420954

Other Notes

Small volumes of anti-FCGR2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR2B. It may not necessarily be applicable to this product.

NCBI GI #
50511930
NCBI GeneID
2213
NCBI Accession #
NP_003992.3
NCBI Related Accession #
NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1
UniProt Secondary Accession #
O95649; Q53X85; Q5VXA9; Q8NIA1; A6H8N3
UniProt Related Accession #
P31994
GenBank Nucleotide #
NM_004001.4
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-b isoform 1
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIb
NCBI Official Symbol
FCGR2B
NCBI Official Synonym Symbols
CD32; FCG2; CD32B; FCGR2; IGFR2
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-b
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD_antigen: CD32
Protein Family
Low affinity immunoglobulin gamma Fc region receptor
UniProt Gene Name
FCGR2B
UniProt Synonym Gene Names
CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
UniProt Entry Name
FCG2B_HUMAN
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-b

NCBI Summary for FCGR2B

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
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UniProt Comments for FCGR2B

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Cell surface; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane

Molecular Function: protein binding

Biological Process: immune response; regulation of immune response; signal transduction

Disease: Malaria, Susceptibility To; Systemic Lupus Erythematosus
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Research Articles on FCGR2B

1. FcgammaRIIB requires Btk and p38 MAPK to mediate antigen-independent inhibition in human B cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FCGR2B antibody

 Reference Product  PubMed Publications
 FCGR3A antibody  >32 publications with FCGR2B and FCGR3A
 INPPL1 antibody  >5 publications with FCGR2B and INPPL1
 INPP5D antibody  >5 publications with FCGR2B and INPP5D
 LYN antibody  >2 publications with FCGR2B and LYN
 FCER1A antibody  >1 publications with FCGR2B and FCER1A
 FCER1G antibody  >1 publications with FCGR2B and FCER1G

Pathways associated with anti-FCGR2B antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Diseases associated with anti-FCGR2B antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >147 publications with FCGR2B and Autoimmune Diseases
 Lupus Erythematosus, Systemic Antibodies  >73 publications with FCGR2B and Lupus Erythematosus, Systemic
 Inflammation Antibodies  >56 publications with FCGR2B and Inflammation
 Disease Models, Animal Antibodies  >52 publications with FCGR2B and Disease Models, Animal
 Kidney Diseases Antibodies  >29 publications with FCGR2B and Kidney Diseases
 Necrosis Antibodies  >23 publications with FCGR2B and Necrosis
 Nervous System Diseases Antibodies  >21 publications with FCGR2B and Nervous System Diseases
 Lung Diseases Antibodies  >14 publications with FCGR2B and Lung Diseases
 Anemia Antibodies  >6 publications with FCGR2B and Anemia
 Malaria Antibodies  >5 publications with FCGR2B and Malaria

Organs/Tissues associated with anti-FCGR2B antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >210 publications with FCGR2B and Blood
 Spleen Antibodies  >31 publications with FCGR2B and Spleen
 Kidney Antibodies  >22 publications with FCGR2B and Kidney
 Lymph Node Antibodies  >22 publications with FCGR2B and Lymph Node
 Bone Marrow Antibodies  >19 publications with FCGR2B and Bone Marrow
 Brain Antibodies  >14 publications with FCGR2B and Brain
 Lung Antibodies  >10 publications with FCGR2B and Lung
 Liver Antibodies  >6 publications with FCGR2B and Liver
 Skin Antibodies  >4 publications with FCGR2B and Skin
 Connective Tissue Antibodies  >3 publications with FCGR2B and Connective Tissue
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