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KLK2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420955

MBS420955 is an antibody targeting KLK2. Also known as Goat anti-KLK2 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLK2, Polyclonal Antibody
Full Product Name
Goat anti-KLK2 Antibody
Product Gene Name
Product Synonym Names
KLK2; kallikrein-related peptidase 2; KLK2A2; MGC12201; hK2; glandular kallikrein 2; kallikrein 2, prostatic; KLK2 antibody; kallikrein-related peptidase 2 antibody; KLK2A2 antibody; MGC12201 antibody; hK2 antibody; glandular kallikrein 2 antibody; kallikrein 2; prostatic antibody
Immunogen Sequence
KHQSLRPDED SSHD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
KLK2 peptide (MBS425847) is used for blocking the activity of KLK2 antibody (MBS420955)
Molecular Weight
17,301 Da
OMIM
147960
Immunogen
Peptide with sequence C-KHQSLRPDEDSSHD, from the internal region of the protein sequence according to NP_005542.1; NP_001002231.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_005542.1; NP_001002231.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-KLK2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-KLK2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Prostate shows vesicular staining in the secretory cells. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx 22kDa band observed in Human Prostate lysates (calculated MW of 24.7kDa according to NP_001002231.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-KLK2 antibody

(0.1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-KLK2 Polyclonal Antibody – Cat# MBS420955

Immunohistochemistry (IHC) of anti-KLK2 antibody

(3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-KLK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420955

Other Notes

Small volumes of anti-KLK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KLK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
kallikrein-2 isoform 1 preproprotein
NCBI Official Synonym Full Names
kallikrein related peptidase 2
NCBI Official Symbol
KLK2
NCBI Official Synonym Symbols
hK2; hGK-1; KLK2A2
UniProt Protein Name
Kallikrein-2
UniProt Synonym Protein Names
Glandular kallikrein-1; hGK-1; Tissue kallikrein-2
Protein Family
UniProt Gene Name
KLK2
UniProt Synonym Gene Names
hGK-1
UniProt Entry Name
KLK2_HUMAN
NCBI Protein Information
kallikrein-2

NCBI Summary for KLK2

This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]
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UniProt Comments for KLK2

KLK2: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.35; Protease

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KLK2 antibody

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