Goat Anti-SIPA1 Polyclonal Antibody – Cat# MBS420982

Staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

SPA1/SIPA1 (SIPA1) Antibody – Goat Polyclonal

Name: SIPA1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420982
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420982

MBS420982 is an antibody targeting SIPA1. Also known as Goat anti-SPA1/SIPA1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPA1/SIPA1, Polyclonal Antibody

Full Product Name

Goat anti-SPA1/SIPA1 Antibody

Product Gene Name

anti-SIPA1 antibody

Product Synonym Gene Name

SPA1

Product Synonym Names

SIPA1; SPA1; MGC17037; signal-induced proliferation-associated gene 1; p130 SPA-1; GTPase-activating protein Spa-1; signal-induced proliferation-associated 1; MGC102688; signal-induced proliferation-associated gene 1; signal-induced proliferation-associat; SIPA1 antibody; SPA1 antibody; MGC17037 antibody; signal-induced proliferation-associated gene 1 antibody; p130 SPA-1 antibody; GTPase-activating protein Spa-1 antibody; signal-induced proliferation-associated 1 antibody; MGC102688 antibody; signal-induced proliferation-associated gene 1 antibody; signal-induced proliferation-associated protein 1 antibody; SPA1 / SIPA1

Immunogen Sequence

ASKQLGSPTA DLA

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

SPA1 / SIPA1 peptide (MBS425748) is used for blocking the activity of SPA1/SIPA1 antibody (MBS420982)

Molecular Weight

112,149 Da

OMIM

602180

Immunogen

Peptide with sequence C-ASKQLGSPTADLA, from the C Terminus of the protein sequence according to NP_694985.28; NP_006738.3.

Epitope

C Terminus

Note

Both variants represent identical protein (NP_694985.28 and NP_006738.3).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-SIPA1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-SIPA1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx.150kDa band observed in Human lysates of lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem.1997 Oct 31;272(44): 28081-8; PMID: 9346962). Recommended concentration: 1-3ug/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

Other Notes

Small volumes of anti-SIPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIPA1. It may not necessarily be applicable to this product.

NCBI GI #

24497629

NCBI GeneID

6494

NCBI Accession #

NP_694985.28

NCBI Related Accession #

NP_006738.3

UniProt Secondary Accession #

O14518; O60484; O60618; Q2YD83

UniProt Related Accession #

Q96FS4

GenBank Nucleotide #

NM_153253.28

NCBI Official Full Name

signal-induced proliferation-associated protein 1

NCBI Official Synonym Full Names

signal-induced proliferation-associated 1

NCBI Official Symbol

SIPA1

NCBI Official Synonym Symbols

SPA1

UniProt Protein Name

Signal-induced proliferation-associated protein 1

UniProt Synonym Protein Names

GTPase-activating protein Spa-1; p130 SPA-1

Protein Family

Signal-induced proliferation-associated protein

UniProt Gene Name

SIPA1

UniProt Synonym Gene Names

SPA1; Sipa-1

UniProt Entry Name

SIPA1_HUMAN

NCBI Protein Information

signal-induced proliferation-associated protein 1

NCBI Summary for SIPA1

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

UniProt Comments for SIPA1

SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.

Protein type: GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytosol; membrane; nucleus; perinuclear region of cytoplasm; protein complex; transport vesicle

Molecular Function: GTPase activator activity; protein binding; protein C-terminus binding

Biological Process: cell proliferation; cellular response to water deprivation; cytoskeleton organization and biogenesis; negative regulation of cell adhesion; negative regulation of cell cycle; negative regulation of cell growth; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction

Research Articles on SIPA1

1. Our results indicate, for the first time, that the SIPA1 -313A>G may have a prognostic role in unresected NSCLC making it a potential predictor of poor survival due to earlier progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SIPA1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
MAPK Cascade Pathway antibodies	MAPK Cascade Pathway Diagram
Rap1 Signaling Pathway antibodies	Rap1 Signaling Pathway Diagram
Rap1 Signaling Pathway antibodies	Rap1 Signaling Pathway Diagram
Rap1 Signalling Pathway antibodies	Rap1 Signalling Pathway Diagram

Diseases associated with anti-SIPA1 antibody

Disease Name	Pubmed Publications
Death Antibodies	>4 publications with SIPA1 and Death
Neoplasm Invasiveness Antibodies	>3 publications with SIPA1 and Neoplasm Invasiveness
Lung Diseases Antibodies	>2 publications with SIPA1 and Lung Diseases
Kidney Diseases Antibodies	>2 publications with SIPA1 and Kidney Diseases
Fetal Death Antibodies	>2 publications with SIPA1 and Fetal Death
Uterine Cervical Neoplasms Antibodies	>1 publications with SIPA1 and Uterine Cervical Neoplasms
Nephritis Antibodies	>1 publications with SIPA1 and Nephritis
Memory Disorders Antibodies	>1 publications with SIPA1 and Memory Disorders
Prenatal Exposure Delayed Effects Antibodies	>1 publications with SIPA1 and Prenatal Exposure Delayed Effects
Inflammation Antibodies	>1 publications with SIPA1 and Inflammation

Organs/Tissues associated with anti-SIPA1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with SIPA1 and Blood
Lymph Node Antibodies	>10 publications with SIPA1 and Lymph Node
Lung Antibodies	>3 publications with SIPA1 and Lung
Thymus Antibodies	>3 publications with SIPA1 and Thymus
Kidney Antibodies	>3 publications with SIPA1 and Kidney
Vascular Antibodies	>2 publications with SIPA1 and Vascular
Eye Antibodies	>2 publications with SIPA1 and Eye
Bone Marrow Antibodies	>2 publications with SIPA1 and Bone Marrow
Brain Antibodies	>2 publications with SIPA1 and Brain
Spleen Antibodies	>1 publications with SIPA1 and Spleen