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MARK4L (MARK4) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421011

MBS421011 is an antibody targeting MARK4. Also known as Goat anti-MARK4L Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MARK4L, Polyclonal Antibody
Full Product Name
Goat anti-MARK4L Antibody
Product Gene Name
Product Synonym Gene Name
MARK4L
Product Synonym Names
MARK4; MAP/microtubule affinity-regulating kinase 4; MARKL1; KIAA1860; Nbla00650; MARK4 serine/threonine protein kinase; MAP/microtubule affinity-regulating kinase like 1; FLJ90097; putative protein product of Nbla00650; MARK4 antibody; MAP/microtubule affinity-regulating kinase 4 antibody; MARKL1 antibody; KIAA1860 antibody; Nbla00650 antibody; MARK4 serine/threonine protein kinase antibody; MAP/microtubule affinity-regulating kinase like 1 antibody; FLJ90097 antibody; putative protein product of Nbla00650 antibody
Immunogen Sequence
IKRKPPPGCS DSPGV
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MARK4L peptide (MBS425229) is used for blocking the activity of MARK4L antibody (MBS421011)
Molecular Weight
75,321 Da
OMIM
606495
Immunogen
Peptide with sequence IKRKPPPGCSDSPGV, from the C Terminus of the protein sequence according to NP_113605.2.
Epitope
C Terminus
Note
This antibody is expected to recognize the reported long isoform 1 (NP_113605.2) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MARK4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MARK4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70kDa band observed in human prostate lysates (calculated MW of 75.3kDa according to NP_113605.2). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-MARK4 antibody

(0.5ug/ml) staining of human prostate lysate (35ug protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-MARK4 Polyclonal Antibody – Cat# MBS421011

Other Notes

Small volumes of anti-MARK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARK4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MAP/microtubule affinity-regulating kinase 4 isoform 2
NCBI Official Synonym Full Names
microtubule affinity regulating kinase 4
NCBI Official Symbol
MARK4
NCBI Official Synonym Symbols
MARK4L; MARK4S; MARKL1; PAR-1D; MARKL1L
UniProt Protein Name
MAP/microtubule affinity-regulating kinase 4
UniProt Synonym Protein Names
MAP/microtubule affinity-regulating kinase-like 1
UniProt Gene Name
MARK4
UniProt Synonym Gene Names
KIAA1860; MARKL1
UniProt Entry Name
MARK4_HUMAN
NCBI Protein Information
MAP/microtubule affinity-regulating kinase 4

NCBI Summary for MARK4

This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for MARK4

MARK4: a member of the CAMKL family of protein kinases. Normally expressed in neural progenitors and is re-expressed in gliomas.

Protein type: CAMK group; CAMKL family; EC 2.7.11.1; Kinase, protein; MARK subfamily; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: centrosome; cytosol; microtubule cytoskeleton; microtubule organizing center; neuron projection

Molecular Function: gamma-tubulin binding; microtubule binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity

Biological Process: microtubule bundle formation; microtubule cytoskeleton organization and biogenesis; nervous system development; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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