Goat Anti-RNASEN Polyclonal Antibody – Cat# MBS421018

(0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RNASEN Antibody – Goat Polyclonal

Name: RNASEN Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421018
Price: 445 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421018

MBS421018 is an antibody targeting RNASEN. Also known as Goat anti-RNASEN/Drosha Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

RNASEN/Drosha, Polyclonal Antibody

Full Product Name

Goat anti-RNASEN/Drosha Antibody

Product Gene Name

anti-RNASEN antibody

Product Synonym Names

Rnasen; Drosha; ribonuclease III, nuclear; 1110013A17Rik; AI874853; Etohi2; MGC115770; Rn3; ethanol induced 2; ribonuclease III; Rnasen antibody; Drosha antibody; ribonuclease III; nuclear antibody; 1110013A17Rik antibody; AI874853 antibody; Etohi2 antibody; MGC115770 antibody; Rn3 antibody; ethanol induced 2 antibody; ribonuclease III antibody; Drosha; RNASEN / Drosha

Immunogen Sequence

KQTDKQKLAQ RE

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

RNASEN / Drosha peptide (MBS425215) is used for blocking the activity of RNASEN/Drosha antibody (MBS421018)

Molecular Weight

158,828 Da

Immunogen

Peptide with sequence C-KQTDKQKLAQRE, from the internal region of the protein sequence according to NP_081075.2.

Epitope

Internal region

Note

This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-RNASEN antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-RNASEN antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 150-160kDa band observed in Human Liver lysates (calculated MW of 151kDa according to NP_081075.2). Recommended concentration: 0.3-1ug/ml.

Other Notes

Small volumes of anti-RNASEN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RNASEN. It may not necessarily be applicable to this product.

NCBI GI #

54873615

NCBI GeneID

14000

NCBI Accession #

NP_081075.2

UniProt Related Accession #

Q5HZJ0

GenBank Nucleotide #

NM_026799.2

NCBI Official Full Name

ribonuclease III

NCBI Official Synonym Full Names

drosha, ribonuclease type III

NCBI Official Symbol

Drosha

NCBI Official Synonym Symbols

Rn3; Etohi2; Rnasen; AI874853; 1110013A17Rik

UniProt Protein Name

Ribonuclease 3

UniProt Synonym Protein Names

Protein Drosha; Ribonuclease III; RNase III

UniProt Gene Name

Drosha

UniProt Synonym Gene Names

Etohi2; Rn3; Rnasen; RNase III

UniProt Entry Name

RNC_MOUSE

NCBI Protein Information

ribonuclease 3

UniProt Comments for RNASEN

DROSHA: a nuclear type-III RNase that, along with the DGCR8, executes the initial step of microRNA (miRNA) processing in the nucleus. Cleaves the local hairpin structures embedded in long primary microRNA transcripts (pri-miRNAs) into pre-miRNAs. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Interacts with Sp1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Ribonuclease; RNA-binding; RNA processing; EC 3.1.26.3

Cellular Component: nucleus

Molecular Function: double-stranded RNA binding; endonuclease activity; endoribonuclease activity; hydrolase activity; lipopolysaccharide binding; metal ion binding; nuclease activity; protein binding; protein homodimerization activity; ribonuclease III activity; RNA binding

Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; pre-microRNA processing; primary microRNA processing; regulation of gene expression; ribosome biogenesis and assembly; RNA interference, production of siRNA; RNA processing; rRNA catabolic process

Research Articles on RNASEN

1. These data indicate that oocyte DICER expression in the fetal ovary is required, and oocyte DROSHA is dispensable, for postnatal follicular development and female fertility in adulthood.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNASEN antibody

Reference Product	PubMed Publications
Dgcr8 antibody	>232 publications with RNASEN and Dgcr8
Eif2c2 antibody	>131 publications with RNASEN and Eif2c2
Eif2c1 antibody	>38 publications with RNASEN and Eif2c1
Xpo5 antibody	>37 publications with RNASEN and Xpo5
Trp53 antibody	>22 publications with RNASEN and Trp53
Ddx5 antibody	>15 publications with RNASEN and Ddx5
Hnrnpa1 antibody	>3 publications with RNASEN and Hnrnpa1
Polr2a antibody	>1 publications with RNASEN and Polr2a
Polr2f antibody	>1 publications with RNASEN and Polr2f

Pathways associated with anti-RNASEN antibody

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Gene Silencing By RNA Pathway antibodies	Gene Silencing By RNA Pathway Diagram
MicroRNA (miRNA) Biogenesis Pathway antibodies	MicroRNA (miRNA) Biogenesis Pathway Diagram
Proteoglycans In Cancer Pathway antibodies	Proteoglycans In Cancer Pathway Diagram
Proteoglycans In Cancer Pathway antibodies	Proteoglycans In Cancer Pathway Diagram
Ribosome Biogenesis In Eukaryotes Pathway antibodies	Ribosome Biogenesis In Eukaryotes Pathway Diagram
Ribosome Biogenesis In Eukaryotes Pathway antibodies	Ribosome Biogenesis In Eukaryotes Pathway Diagram

Diseases associated with anti-RNASEN antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>79 publications with RNASEN and Nervous System Diseases
Disease Models, Animal Antibodies	>73 publications with RNASEN and Disease Models, Animal
Cardiovascular Diseases Antibodies	>61 publications with RNASEN and Cardiovascular Diseases
Inflammation Antibodies	>57 publications with RNASEN and Inflammation
Adenocarcinoma Antibodies	>57 publications with RNASEN and Adenocarcinoma
Liver Diseases Antibodies	>43 publications with RNASEN and Liver Diseases
Brain Diseases Antibodies	>41 publications with RNASEN and Brain Diseases
Genital Diseases, Male Antibodies	>40 publications with RNASEN and Genital Diseases, Male
Necrosis Antibodies	>28 publications with RNASEN and Necrosis
Infertility, Male Antibodies	>19 publications with RNASEN and Infertility, Male

Organs/Tissues associated with anti-RNASEN antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>144 publications with RNASEN and Brain
Kidney Antibodies	>96 publications with RNASEN and Kidney
Muscle Antibodies	>89 publications with RNASEN and Muscle
Lung Antibodies	>87 publications with RNASEN and Lung
Embryonic Tissue Antibodies	>86 publications with RNASEN and Embryonic Tissue
Heart Antibodies	>78 publications with RNASEN and Heart
Bone Antibodies	>46 publications with RNASEN and Bone
Ovary Antibodies	>45 publications with RNASEN and Ovary
Eye Antibodies	>41 publications with RNASEN and Eye
Skin Antibodies	>39 publications with RNASEN and Skin