• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GRB2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421084

MBS421084 is an antibody targeting GRB2. Also known as Goat anti-GRB2 Antibody. Reactive in Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRB2, Polyclonal Antibody
Full Product Name
Goat anti-GRB2 Antibody
Product Gene Name
Product Synonym Names
GRB2; growth factor receptor-bound protein 2; ASH; ASH protein; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; HT027; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3; NCKAP2; OTTHUMP00000166096; GRB2 antibody; growth factor receptor-bound protein 2 antibody; ASH antibody; ASH protein antibody; EGFRBP-GRB2 antibody; Grb3-3 antibody; MST084 antibody; MSTP084 antibody; HT027 antibody; abundant SRC homology antibody; epidermal growth factor receptor-binding protein GRB2 antibody; growth factor receptor-bound protein 3 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; abundant SRC homology antibody
Immunogen Sequence
PRNYVTPVNR NV
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Matching Pairs
Unconjugated Antibody: GRB2 (MBS421084)
Biotin Conjugated Antibody: GRB2 (MBS423553)
Antibody/Peptide Pairs
GRB2 peptide (MBS426749) is used for blocking the activity of GRB2 antibody (MBS421084)
Molecular Weight
20,557 Da
OMIM
108355
Immunogen
Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GRB2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GRB2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Data obtained by previous batch (different goat). Recommended concentration, 0.1-0.3ug/ml. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained.
Western Blot: Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15ug/ml.

Western Blot (WB) of anti-GRB2 antibody

(0. 05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-GRB2 Polyclonal Antibody – Cat# MBS421084

Western Blot (WB) of anti-GRB2 antibody

(0. 03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GRB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421084

Western Blot (WB) of anti-GRB2 antibody

(0. 05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-GRB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421084

Immunohistochemistry (IHC) of anti-GRB2 antibody

(0.3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
Goat Anti-GRB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421084

Other Notes

Small volumes of anti-GRB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
growth factor receptor-bound protein 2 isoform 1
NCBI Official Synonym Full Names
growth factor receptor bound protein 2
NCBI Official Symbol
GRB2
NCBI Official Synonym Symbols
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
UniProt Protein Name
Growth factor receptor-bound protein 2
UniProt Synonym Protein Names
Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2
UniProt Gene Name
GRB2
UniProt Synonym Gene Names
ASH
UniProt Entry Name
GRB2_HUMAN
NCBI Protein Information
growth factor receptor-bound protein 2

NCBI Summary for GRB2

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for GRB2

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000