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Neudesin/NENF (NENF) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421096

MBS421096 is an antibody targeting NENF. Also known as Goat anti-Neudesin/NENF Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Neudesin/NENF, Polyclonal Antibody
Full Product Name
Goat anti-Neudesin/NENF Antibody
Product Gene Name
Product Synonym Names
NENF; neuron derived neurotrophic factor; CIR2; SCIRP10; SPUF; SCIRP10-related protein; Spinal cord injury related protein 10; cell growth-inhibiting protein 47 neudesin secreted protein of unknown function; Neudesin; NENF antibody; neuron derived neurotrophic factor antibody; CIR2 antibody; SCIRP10 antibody; SPUF antibody; SCIRP10-related protein antibody; Spinal cord injury related protein 10 antibody; cell growth-inhibiting protein 47 neudesin secreted protein of unknown function antibody; Neudesin antibody; Neudesin; Neudesin / NENF
Immunogen Sequence
NEDGSPNLDF KP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
18,856 Da
OMIM
611874
Immunogen
Peptide with sequence C-NEDGSPNLDFKP, from the internal region (near the C Terminus) of the protein sequence according to NP_037481.1.
Epitope
Internal region (near the C Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NENF antibody
Peptide ELISA (EIA)
Application Notes for anti-NENF antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NENF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NENF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neudesin
NCBI Official Synonym Full Names
neudesin neurotrophic factor
NCBI Official Symbol
NENF
NCBI Official Synonym Symbols
CIR2; SPUF; SCIRP10
UniProt Protein Name
Neudesin
UniProt Synonym Protein Names
Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47
Protein Family
UniProt Gene Name
NENF
UniProt Synonym Gene Names
CIR2; SPUF; SPUF protein
UniProt Entry Name
NENF_HUMAN
NCBI Protein Information
neudesin

NCBI Summary for NENF

This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2009]

UniProt Comments for NENF

NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: endomembrane system; extracellular space; membrane; nucleoplasm; nucleus

Molecular Function: growth factor activity; metal ion binding

Biological Process: negative regulation of appetite; positive regulation of MAPKKK cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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