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Duffy/Fy/DARC (DARC) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421099

MBS421099 is an antibody targeting DARC. Also known as Goat anti-Duffy/FY/DARC Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Duffy/FY/DARC, Polyclonal Antibody
Full Product Name
Goat anti-Duffy/FY/DARC Antibody
Product Gene Name
anti-DARC antibody
Product Synonym Gene Name
Duffy; FY
Product Synonym Names
FY; DARC; HGNC: 4035; Duffy blood group; CCBP1; GPD; CTA-134P22.3; CD234; FY; OTTHUMP00000060081; Duffy blood group, chemokine receptor; Duffy blood group antigen; Glycoprotein D; Dfy; GpFy; WBCQ1; Fy glycoprotein; OTTHUMP00000035293; OTTHUMP00000035294; FY antibody; DARC antibody; HGNC: 4035 antibody; Duffy blood group antibody; CCBP1 antibody; GPD antibody; CTA-134P22.3 antibody; CD234 antibody; FY antibody; OTTHUMP00000060081 antibody; Duffy blood group; chemokine receptor antibody; Duffy blood group antigen antibody; Glycoprotein D antibody; Dfy antibody; GpFy antibody; WBCQ1 antibody; Fy glycoprotein antibody; OTTHUMP00000035293 antibody; OTTHUMP00000035294 antibody; Duffy / FY / DARC
Immunogen Sequence
HRAELSPSTE NSSQLDFED
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Matching Pairs
Unconjugated Antibody: Duffy/FY/DARC (MBS421099)
Biotin Conjugated Antibody: Duffy/FY/DARC (MBS423711)
Antibody/Peptide Pairs
Duffy / FY / DARC peptide (MBS426067) is used for blocking the activity of Duffy/FY/DARC antibody (MBS421099)
Molecular Weight
35,678 Da
OMIM
110700
Immunogen
Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_001116423.1; NP_002027.2.
Epitope
N Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-DARC antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-DARC antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Human Lung showing endothelial staining. Recommended concentration, 3ug/ml.
Western Blot: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3ug/ml.
Flow cytometry: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Western Blot (WB) of anti-DARC antibody

(0.1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DARC Polyclonal Antibody – Cat# MBS421099

Immunohistochemistry (IHC) of anti-DARC antibody

(3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-DARC Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421099

Other Notes

Small volumes of anti-DARC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DARC. It may not necessarily be applicable to this product.

NCBI GI #
171846251
NCBI GeneID
2532
NCBI Accession #
NP_001116423.1
NCBI Related Accession #
NP_002027.2
UniProt Secondary Accession #
O75898; Q16300; Q8WWE3; Q9UJP0; Q9UKZ5; Q9UKZ6; Q9UQE1; A8YPG5
UniProt Related Accession #
Q16570
GenBank Nucleotide #
NM_001122951.2
NCBI Official Full Name
atypical chemokine receptor 1 isoform a
NCBI Official Synonym Full Names
atypical chemokine receptor 1 (Duffy blood group)
NCBI Official Symbol
ACKR1
NCBI Official Synonym Symbols
FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1
UniProt Protein Name
Atypical chemokine receptor 1
UniProt Synonym Protein Names
Duffy antigen/chemokine receptor; Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor; CD_antigen: CD234
UniProt Gene Name
ACKR1
UniProt Synonym Gene Names
DARC; FY; GPD; GpFy
UniProt Entry Name
ACKR1_HUMAN
NCBI Protein Information
atypical chemokine receptor 1

NCBI Summary for DARC

The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DARC

DARC: Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Belongs to the G-protein coupled receptor Duffy family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, Duffy family; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: early endosome; integral to membrane; plasma membrane; recycling endosome

Molecular Function: C-C chemokine binding; G-protein coupled receptor activity; receptor activity; transmembrane receptor activity

Biological Process: defense response; G-protein coupled receptor protein signaling pathway; inflammatory response; regulation of chemokine production

Disease: Blood Group, Duffy System; Malaria, Susceptibility To; White Blood Cell Count Quantitative Trait Locus 1
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Research Articles on DARC

1. the odds for neutropenia in the ACKR1-null (FY-) individuals were 46-fold higher than for FY+ individuals (Crude odds ratio = 46, 95% confidence interval = 8.03-263, P < 0.001)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DARC antibody

 Reference Product  PubMed Publications
 DARC antibody  >7 publications with DARC and DARC
 CCL2 antibody  >2 publications with DARC and CCL2
 CCL7 antibody  >1 publications with DARC and CCL7
 CXCL1 antibody  >1 publications with DARC and CXCL1

Pathways associated with anti-DARC antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-DARC antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with DARC and Inflammation
 Brain Diseases Antibodies  >1 publications with DARC and Brain Diseases
 Atherosclerosis Antibodies  >1 publications with DARC and Atherosclerosis
 Lung Diseases Antibodies  >1 publications with DARC and Lung Diseases
 Heart Diseases Antibodies  >1 publications with DARC and Heart Diseases

Organs/Tissues associated with anti-DARC antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with DARC and Heart
 Vascular Antibodies  >1 publications with DARC and Vascular
 Lung Antibodies  >1 publications with DARC and Lung
 Brain Antibodies  >1 publications with DARC and Brain
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