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ARIH1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421133

MBS421133 is an antibody targeting ARIH1. Also known as Goat anti-ARIH1/HHARI Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARIH1/HHARI, Polyclonal Antibody
Full Product Name
Goat anti-ARIH1/HHARI Antibody
Product Gene Name
anti-ARIH1 antibody
Product Synonym Gene Name
HHARI
Product Synonym Names
ARIH1; HHARI; ARI; HARI; UBCH7BP; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila); ariadne homolog; ariadne, Drosophila, homolog of; DKFZp686O13120; FLJ20329; FLJ93118; ariadne ubiquitin-conjugating enzyme E2 binding prote; ARIH1 antibody; HHARI antibody; ARI antibody; HARI antibody; UBCH7BP antibody; ariadne homolog; ubiquitin-conjugating enzyme E2 binding protein; 1 (Drosophila) antibody; ariadne homolog antibody; ariadne; Drosophila; homolog of antibody; DKFZp686O13120 antibody; FLJ20329 antibody; FLJ93118 antibody; ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 antibody; ARIH1 / HHARI
Immunogen Sequence
HEGYEKDLWE YIED
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ARIH1 / HHARI peptide (MBS425220) is used for blocking the activity of ARIH1/HHARI antibody (MBS421133)
Molecular Weight
64,118 Da
OMIM
605624
Immunogen
Peptide with sequence C-HEGYEKDLWEYIED, from the C Terminus of the protein sequence according to NP_005735.2.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ARIH1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ARIH1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1ug/ml.

Western Blot (WB) of anti-ARIH1 antibody

Staining (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-ARIH1 Polyclonal Antibody – Cat# MBS421133

Other Notes

Small volumes of anti-ARIH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARIH1. It may not necessarily be applicable to this product.

NCBI GI #
187761373
NCBI GeneID
25820
NCBI Accession #
NP_005735.2
UniProt Secondary Accession #
O76026; Q9H3T6; Q9UEN0; Q9UP39; B2R6U3
UniProt Related Accession #
Q9Y4X5
GenBank Nucleotide #
NM_005744.3
NCBI Official Full Name
E3 ubiquitin-protein ligase ARIH1
NCBI Official Synonym Full Names
ariadne RBR E3 ubiquitin protein ligase 1
NCBI Official Symbol
ARIH1
NCBI Official Synonym Symbols
ARI; HARI; HHARI; UBCH7BP
UniProt Protein Name
E3 ubiquitin-protein ligase ARIH1
UniProt Synonym Protein Names
H7-AP2; HHARI; Monocyte protein 6; MOP-6; Protein ariadne-1 homolog; ARI-1; UbcH7-binding protein; UbcM4-interacting protein; Ubiquitin-conjugating enzyme E2-binding protein 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
ARIH1
UniProt Synonym Gene Names
ARI; MOP6; UBCH7BP; MOP-6; ARI-1
UniProt Entry Name
ARI1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase ARIH1

UniProt Comments for ARIH1

ARIH1: E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Belongs to the RBR family. Ariadne subfamily.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: cytoplasm; cytosol; ubiquitin ligase complex

Molecular Function: ligase activity; protein binding; small conjugating protein ligase activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; ubiquitin-dependent protein catabolic process
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Research Articles on ARIH1

1. DNA damage induces an increase in ARIH1 protein levels and association of ARIH1 with 4EHP. In turn, this causes 4EHP recruitment to the mRNA cap, where it is known to compete with eIF4E.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARIH1 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram

Diseases associated with anti-ARIH1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with ARIH1 and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with ARIH1 and Brain Diseases
 Movement Disorders Antibodies  >3 publications with ARIH1 and Movement Disorders
 Carcinoma, Hepatocellular Antibodies  >1 publications with ARIH1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with ARIH1 and Liver Neoplasms
 Adenocarcinoma Antibodies  >1 publications with ARIH1 and Adenocarcinoma
 Liver Diseases Antibodies  >1 publications with ARIH1 and Liver Diseases
 Weight Loss Antibodies  >1 publications with ARIH1 and Weight Loss

Organs/Tissues associated with anti-ARIH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with ARIH1 and Brain
 Liver Antibodies  >1 publications with ARIH1 and Liver
 Testis Antibodies  >1 publications with ARIH1 and Testis
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