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MAD3/MXD3 (MXD3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421141

MBS421141 is an antibody targeting MXD3. Also known as Goat anti-MAD3/MXD3 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human,. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAD3/MXD3, Polyclonal Antibody
Full Product Name
Goat anti-MAD3/MXD3 Antibody
Product Gene Name
Product Synonym Gene Name
MAD3
Product Synonym Names
MXD3; MAD3; MAX dimerization protein 3; MGC2383; hypothetical protein MGC2383; likely ortholog of mouse Max dimerization protein 3; BHLHC13; FLJ35523; MYX; Max-associated protein 3; Max-interacting transcriptional repressor MAD3; MXD3 antibody; MAD3 antibody; MAX dimerization protein 3 antibody; MGC2383 antibody; hypothetical protein MGC2383 antibody; likely ortholog of mouse Max dimerization protein 3 antibody; BHLHC13 antibody; FLJ35523 antibody; MYX antibody; Max-associated protein 3 antibody; Max-interacting transcriptional repressor MAD3 antibody; MAD3 / MXD3
Immunogen Sequence
QEHSYSHGGG AWL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human,
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
42,821 Da
OMIM
609450
Immunogen
Peptide with sequence C-QEHSYSHGGGAWL, from the C Terminus of the protein sequence according to NP_112590.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoform a (NP_112590.1) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MXD3 antibody
Peptide ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-MXD3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: An anonymous customer found positive results in IHC on Human HeLa cells.
Western Blot: No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3ug/ml.
Immunofluorescence: This product has been successfully used by an anonymous customer on methanol-fixed and 0.5% TX100-permeabilized HeLa cells, staning the cytoplasm.

Other Notes

Small volumes of anti-MXD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MXD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
max dimerization protein 3 isoform a
NCBI Official Synonym Full Names
MAX dimerization protein 3
NCBI Official Symbol
MXD3
NCBI Official Synonym Symbols
MYX; MAD3; BHLHC13
UniProt Protein Name
Max dimerization protein 3
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 13; bHLHc13; Max-associated protein 3; Max-interacting transcriptional repressor MAD3; Myx
UniProt Gene Name
MXD3
UniProt Synonym Gene Names
BHLHC13; MAD3; Max dimerizer 3; bHLHc13
UniProt Entry Name
MAD3_HUMAN
NCBI Protein Information
max dimerization protein 3

NCBI Summary for MXD3

This gene encodes a member of the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. The encoded protein forms a heterodimer with the cofactor MAX which binds specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disruption of the MAX-MXD3 complex is associated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of this gene encoding different isoforms have been described.[provided by RefSeq, Dec 2008]
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UniProt Comments for MXD3

MXD3: Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; protein dimerization activity

Biological Process: negative regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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