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NCF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421184

MBS421184 is an antibody targeting NCF1. Also known as Goat anti-NCF1/p47phox Antibody. Reactive in Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Rabbit, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NCF1/p47phox, Polyclonal Antibody
Full Product Name
Goat anti-NCF1/p47phox Antibody
Product Gene Name
anti-NCF1 antibody
Product Synonym Gene Name
p47phox
Product Synonym Names
Goat Anti-p47phox (mouse) Antibody; NCF1; p47phox; FLJ79451; NCF1A; NOXO2; SH3PXD1A; neutrophil cytosolic factor 1; Neutrophil cytosolic factor-1 (47kD); neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1); NADPH oxidase organizer 2; NCF1 antibody; p47phox antibody; FLJ79451 antibody; NCF1A antibody; NOXO2 antibody; SH3PXD1A antibody; neutrophil cytosolic factor 1 antibody; Neutrophil cytosolic factor-1 (47kD) antibody; neutrophil cytosolic factor 1 (47kD; chronic granulomatous disease; autosomal 1) antibody; NADPH oxidase organizer 2 antibody; NCF1 / p47phox
Immunogen Sequence
SESTKRKLAS AV
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Rabbit, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NCF1 / p47phox peptide (MBS426586) is used for blocking the activity of NCF1/p47phox antibody (MBS421184)
Molecular Weight
30,781 Da
OMIM
233700
Immunogen
Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
Epitope
C Terminus
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7660
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NCF1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NCF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 20000.
Western Blot: Approx 48kDa band observed in lysates of cell line U937 and of Mouse Thymus, and 50kDa observed in Pig Spleen lysates (calculated MW of 44.7kDa according to NP_000256.3). Recommended concentration: 0.5-1.5ug/ml.

Western Blot (WB) of anti-NCF1 antibody

(0.2ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NCF1 Polyclonal Antibody – Cat# MBS421184

Western Blot (WB) of anti-NCF1 antibody

1ug/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NCF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421184

Other Notes

Small volumes of anti-NCF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCF1. It may not necessarily be applicable to this product.

NCBI GI #
115298672
NCBI GeneID
653361
NCBI Accession #
NP_000256.3
UniProt Secondary Accession #
O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
UniProt Related Accession #
P14598
GenBank Nucleotide #
NM_000265.4
NCBI Official Full Name
neutrophil cytosol factor 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; SH3PXD1A
UniProt Protein Name
Neutrophil cytosol factor 1
UniProt Synonym Protein Names
47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Protein Family
Neutrophil cytosol factor
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A; NCF-1
UniProt Entry Name
NCF1_HUMAN
NCBI Protein Information
neutrophil cytosol factor 1

NCBI Summary for NCF1

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

UniProt Comments for NCF1

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex

Molecular Function: electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; cellular defense response; innate immune response; protein targeting to membrane; respiratory burst; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I
Show more

Research Articles on NCF1

1. increased levels of gp91phox, p47phox and p22phox likely account for the interferon-gamma mediated enhancement of dimethyl sulfoxide-induced Nox2 activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NCF1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway antibodies  Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-NCF1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >58 publications with NCF1 and Inflammation
 Arthritis, Rheumatoid Antibodies  >28 publications with NCF1 and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >12 publications with NCF1 and Cardiovascular Diseases
 Necrosis Antibodies  >8 publications with NCF1 and Necrosis
 Heart Diseases Antibodies  >8 publications with NCF1 and Heart Diseases
 Diabetes Mellitus Antibodies  >7 publications with NCF1 and Diabetes Mellitus
 Hypertension Antibodies  >6 publications with NCF1 and Hypertension
 Diabetes Mellitus, Experimental Antibodies  >4 publications with NCF1 and Diabetes Mellitus, Experimental
 Fibrosis Antibodies  >4 publications with NCF1 and Fibrosis
 Liver Diseases Antibodies  >3 publications with NCF1 and Liver Diseases

Organs/Tissues associated with anti-NCF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >64 publications with NCF1 and Blood
 Lung Antibodies  >11 publications with NCF1 and Lung
 Lymph Node Antibodies  >9 publications with NCF1 and Lymph Node
 Lymph Antibodies  >8 publications with NCF1 and Lymph
 Spleen Antibodies  >7 publications with NCF1 and Spleen
 Skin Antibodies  >6 publications with NCF1 and Skin
 Brain Antibodies  >4 publications with NCF1 and Brain
 Intestine Antibodies  >3 publications with NCF1 and Intestine
 Connective Tissue Antibodies  >3 publications with NCF1 and Connective Tissue
 Kidney Antibodies  >3 publications with NCF1 and Kidney
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