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FBXW2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421205

MBS421205 is an antibody targeting FBXW2. Also known as Goat anti-FBXW2 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FBXW2, Polyclonal Antibody
Full Product Name
Goat anti-FBXW2 Antibody
Product Gene Name
anti-FBXW2 antibody
Product Synonym Names
FBXW2; F-box and WD-40 domain protein 2; FBW2; Fwd2; MGC117371; Md6; F-box protein Fbw2; F-box and WD repeat domain containing 2; FBXW2 antibody; F-box and WD-40 domain protein 2 antibody; FBW2 antibody; Fwd2 antibody; MGC117371 antibody; Md6 antibody; F-box protein Fbw2 antibody; F-box and WD repeat domain containing 2 antibody
Immunogen Sequence
KRGSSFLAGE HPG
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
FBXW2 peptide (MBS425494) is used for blocking the activity of FBXW2 antibody (MBS421205)
Molecular Weight
44,466 Da
OMIM
609071
Immunogen
Peptide with sequence C-KRGSSFLAGEHPG, from the C Terminus of the protein sequence according to NP_036296.2.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-FBXW2 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-FBXW2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue.
Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48. 0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr.30kDa - both bands were successfully blocked by incubation with the immunising peptide.

Western Blot (WB) of anti-FBXW2 antibody

Staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-FBXW2 Polyclonal Antibody – Cat# MBS421205

Immunohistochemistry (IHC) of anti-FBXW2 antibody

(3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-FBXW2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421205

Other Notes

Small volumes of anti-FBXW2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FBXW2. It may not necessarily be applicable to this product.

NCBI GI #
118402584
NCBI GeneID
26190
NCBI Accession #
NP_036296.2
UniProt Secondary Accession #
Q4VXH2; Q7Z4V6; Q8WV51; Q9HA09; Q9UKA3; B3KRL8
UniProt Related Accession #
Q9UKT8
GenBank Nucleotide #
NM_012164.3
NCBI Official Full Name
F-box/WD repeat-containing protein 2
NCBI Official Synonym Full Names
F-box and WD repeat domain containing 2
NCBI Official Symbol
FBXW2
NCBI Official Synonym Symbols
Md6; FBW2; Fwd2
UniProt Protein Name
F-box/WD repeat-containing protein 2
UniProt Synonym Protein Names
F-box and WD-40 domain-containing protein 2; Protein MD6
Protein Family
F-box/WD repeat-containing protein
UniProt Gene Name
FBXW2
UniProt Synonym Gene Names
FBW2; FWD2
UniProt Entry Name
FBXW2_HUMAN
NCBI Protein Information
F-box/WD repeat-containing protein 2

NCBI Summary for FBXW2

F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. [provided by RefSeq, Jul 2008]
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UniProt Comments for FBXW2

FBXW2: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9q34

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: protein modification process; protein ubiquitination; proteolysis
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Research Articles on FBXW2

1. RACK1 competes with GCM1 for FBW2 and thereby prevents GCM1 ubiquitination, which is also supported by the observation that GCM1 is destabilized in RACK1-knockdown BeWo placental cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FBXW2 antibody

 Products by Pathway  Pathway Diagram
 Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies  Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Protein Folding Pathway antibodies  Protein Folding Pathway Diagram
 Wnt Signaling Pathway antibodies  Wnt Signaling Pathway Diagram
 Wnt Signaling Pathway And Pluripotency antibodies  Wnt Signaling Pathway And Pluripotency Diagram

Diseases associated with anti-FBXW2 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with FBXW2 and Carcinoma
 Lung Diseases Antibodies  >1 publications with FBXW2 and Lung Diseases
 Lung Neoplasms Antibodies  >1 publications with FBXW2 and Lung Neoplasms

Organs/Tissues associated with anti-FBXW2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >3 publications with FBXW2 and Muscle
 Placenta Antibodies  >2 publications with FBXW2 and Placenta
 Heart Antibodies  >2 publications with FBXW2 and Heart
 Testis Antibodies  >2 publications with FBXW2 and Testis
 Lung Antibodies  >2 publications with FBXW2 and Lung
 Parathyroid Antibodies  >1 publications with FBXW2 and Parathyroid
 Liver Antibodies  >1 publications with FBXW2 and Liver
 Uterus Antibodies  >1 publications with FBXW2 and Uterus
 Brain Antibodies  >1 publications with FBXW2 and Brain
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