Goat Anti-CARD16 Polyclonal Antibody – Cat# MBS421219

COP1/PSEUDO-ICE (CARD16) Antibody – Goat Polyclonal

Name: CARD16 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421219
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421219

MBS421219 is an antibody targeting CARD16. Also known as Goat anti-COP1/PSEUDO-ICE (Isofom 2) Antibody. Reactive in Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

COP1/PSEUDO-ICE (CARD16), Polyclonal Antibody

Full Product Name

Goat anti-COP1/PSEUDO-ICE (Isofom 2) Antibody

Product Gene Name

anti-CARD16 antibody

Product Synonym Gene Name

COP1; PSEUDO-ICE

Product Synonym Names

CARD16; COP; CARD only protein; PSEUDO-ICE; COP1; caspase-1 dominant-negative inhibitor pseudo-ICE; pseudo interleukin-1beta converting enzyme; caspase recruitment domain family, member 16; CARD16 antibody; COP antibody; CARD only protein antibody; PSEUDO-ICE antibody; COP1 antibody; caspase-1 dominant-negative inhibitor pseudo-ICE antibody; pseudo interleukin-1beta converting enzyme antibody; caspase recruitment domain family; member 16 antibody; COP1 / PSEUDO-ICE (Isofom 2)

Immunogen Sequence

ETLGLSAGPI PGN

Clonality

Polyclonal

Host

Goat

Species Reactivity

Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

COP1 / PSEUDO-ICE peptide (MBS425386) is used for blocking the activity of COP1/PSEUDO-ICE antibody (MBS421219)

Molecular Weight

10,737 Da

OMIM

615680

Immunogen

Peptide with sequence C-ETLGLSAGPIPGN, from the C Terminus of the protein sequence according to NP_443121.1.

Epitope

C Terminus

Note

This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CARD16 antibody

Peptide ELISA (EIA)

Application Notes for anti-CARD16 antibody

Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments gave an approx 28-30kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7kDa according to NP_443121.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-CARD16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CARD16. It may not necessarily be applicable to this product.

NCBI GI #

16751833

NCBI GeneID

114769

NCBI Accession #

NP_443121.1

UniProt Secondary Accession #

Q96RJ9

UniProt Related Accession #

Q5EG05

GenBank Nucleotide #

NM_052889.2

NCBI Official Full Name

caspase recruitment domain-containing protein 16 isoform 2

NCBI Official Synonym Full Names

caspase recruitment domain family member 16

NCBI Official Symbol

CARD16

NCBI Official Synonym Symbols

COP; COP1; PSEUDO-ICE

UniProt Protein Name

Caspase recruitment domain-containing protein 16

UniProt Synonym Protein Names

Caspase recruitment domain-only protein 1; CARD-only protein 1; Caspase-1 inhibitor COP; Pseudo interleukin-1 beta converting enzyme; Pseudo-ICE; Pseudo-IL1B-converting enzyme

Protein Family

Caspase recruitment domain-containing protein

UniProt Gene Name

CARD16

UniProt Synonym Gene Names

COP; COP1; CARD-only protein 1; Pseudo-ICE; Pseudo-IL1B-converting enzyme

UniProt Entry Name

CAR16_HUMAN

NCBI Protein Information

caspase recruitment domain-containing protein 16

UniProt Comments for CARD16

CARD16: Caspase inhibitor. Acts as a regulator of procaspase- 1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro- inflammatory cytokine response. Also able to inhibit CASP1- mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. Down-regulated in patients suffering of Huntington disease. Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2. 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 11q22

Molecular Function: cysteine protease inhibitor activity

Biological Process: regulation of apoptosis

Research Articles on CARD16

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-CARD16 antibody

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>1 publications with CARD16 and Heart Diseases
Inflammation Antibodies	>1 publications with CARD16 and Inflammation