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SLIMMER/FHL1 Isoform B (FHL1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421232

MBS421232 is an antibody targeting FHL1. Also known as Goat anti-SLIMMER/FHL1 isoform B Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLIMMER/FHL1 isoform B, Polyclonal Antibody
Full Product Name
Goat anti-SLIMMER/FHL1 isoform B Antibody
Product Gene Name
Product Synonym Gene Name
SLIMMER; FHL1
Product Synonym Names
Goat Anti-FHL1 / SLIM1, Biotinylated Antibody; FHL1; KYO-T; SLIM1; bA535K18.1; four and a half LIM domains 1; Four-and-a-half LIM domains 1; bA535K18.1 (LIM protein SLIMMER); RP11-535K18.1; FHL1A; FHL1B; FLH1A; KYOT; MGC111107; SLIMMER, XMPMA; LIM protein SLIMMER; four-and-a-half Lin11, Isl-1 and Mec-; FHL1 antibody; KYO-T antibody; SLIM1 antibody; bA535K18.1 antibody; four and a half LIM domains 1 antibody; Four-and-a-half LIM domains 1 antibody; bA535K18.1 (LIM protein SLIMMER) antibody; RP11-535K18.1 antibody; FHL1A antibody; FHL1B antibody; FLH1A antibody; KYOT antibody; MGC111107 antibody; SLIMMER; antibody; XMPMA antibody; LIM protein SLIMMER antibody; four-and-a-half Lin11 antibody; Isl-1 and Mec-3 domains 1 antibody; skeletal muscle LIM-protein1 antibody; SLIMMER / FHL1 isoform B
Immunogen Sequence
CTVSRVSHPV SKARK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
33,579 Da
OMIM
300163
Immunogen
Peptide with sequence CTVSRVSHPVSKARK, from the internal region of the protein sequence according to AAC72886.1.
Epitope
Internal region
Note
This antibody is specific for the FHL-1 isoform B (SLIMMER) and is expected to recognise an epitope corresponding to aa 233-246 of human SLIMMER protein.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-FHL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-FHL1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 30kDa band observed in Human Heart lysates is consistent with literature (PMID: 10480922; calculated MW of 36.3kDa according to AAC72886). Recommended concentration: 0. 01-0.1ug/ml.

Western Blot (WB) of anti-FHL1 antibody

(0. 01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-FHL1 Polyclonal Antibody – Cat# MBS421232

Other Notes

Small volumes of anti-FHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FHL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
LIM protein SLIMMER
NCBI Official Synonym Full Names
four and a half LIM domains 1
NCBI Official Symbol
FHL1
NCBI Official Synonym Symbols
KYOT; SLIM; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
UniProt Protein Name
Four and a half LIM domains protein 1
UniProt Synonym Protein Names
Skeletal muscle LIM-protein 1; SLIM; SLIM-1
UniProt Gene Name
FHL1
UniProt Synonym Gene Names
SLIM1; FHL-1; SLIM; SLIM-1
UniProt Entry Name
FHL1_HUMAN
NCBI Protein Information
four and a half LIM domains protein 1

NCBI Summary for FHL1

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]
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UniProt Comments for FHL1

May have an involvement in muscle development or hypertrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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