EXO1 Antibody – Goat Polyclonal

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]

EXO1: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: 5'-3' exodeoxyribonuclease activity; 5'-3' exonuclease activity; chromatin binding; DNA binding; double-stranded DNA specific 5'-3' exodeoxyribonuclease activity; exonuclease activity; flap endonuclease activity; metal ion binding; protein binding; ribonuclease H activity; single-stranded DNA specific 5'-3' exodeoxyribonuclease activity

Biological Process: DNA recombination; DNA repair; DNA replication; DNA synthesis during DNA repair; humoral immune response mediated by circulating immunoglobulin; isotype switching; meiotic cell cycle; mismatch repair; somatic hypermutation of immunoglobulin genes; strand displacement