Goat Anti-SAE1 Polyclonal Antibody – Cat# MBS421251

Staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

SAE1 Antibody – Goat Polyclonal

Name: SAE1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421251
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421251

MBS421251 is an antibody targeting SAE1. Also known as Goat anti-SAE1/AOS1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SAE1/AOS1, Polyclonal Antibody

Full Product Name

Goat anti-SAE1/AOS1 Antibody

Product Gene Name

anti-SAE1 antibody

Product Synonym Gene Name

AOS1

Product Synonym Names

SAE1; AOS1; SUA1; HSPC140; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like protein SUMO-1 activating enzyme; SUMO1 activating enzyme subunit 1; FLJ3091; UBLE1A; activator of S; SAE1 antibody; AOS1 antibody; SUA1 antibody; HSPC140 antibody; SUMO-1 activating enzyme subunit 1 antibody; sentrin/SUMO-activating protein AOS1 antibody; SUMO-1 activating enzyme E1 N subunit antibody; ubiquitin-like protein SUMO-1 activating enzyme antibody; SUMO1 activating enzyme subunit 1 antibody; FLJ3091 antibody; UBLE1A antibody; activator of SUMO1 antibody; ubiquitin-like 1-activating enzyme E1A antibody; SAE1 / AOS1

Immunogen Sequence

MKGNGIVECL GPK

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

SAE1 / AOS1 peptide (MBS425498) is used for blocking the activity of SAE1/AOS1 antibody (MBS421251)

Molecular Weight

33,063 Da

OMIM

613294

Immunogen

Peptide with sequence MKGNGIVECLGPK, from the C Terminus of the protein sequence according to NP_005491.1.

Epitope

C Terminus

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-SAE1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-SAE1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1ug/ml.

Other Notes

Small volumes of anti-SAE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #

4885585

NCBI GeneID

10055

NCBI Accession #

NP_005491.1

UniProt Secondary Accession #

O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6

UniProt Related Accession #

Q9UBE0

GenBank Nucleotide #

NM_005500.2

NCBI Official Full Name

SUMO-activating enzyme subunit 1 isoform a

NCBI Official Synonym Full Names

SUMO1 activating enzyme subunit 1

NCBI Official Symbol

SAE1

NCBI Official Synonym Symbols

AOS1; SUA1; UBLE1A; HSPC140

UniProt Protein Name

SUMO-activating enzyme subunit 1

UniProt Synonym Protein Names

Ubiquitin-like 1-activating enzyme E1A

Protein Family

SUMO-activating enzyme

UniProt Gene Name

SAE1

UniProt Synonym Gene Names

AOS1; SUA1; UBLE1A

UniProt Entry Name

SAE1_HUMAN

NCBI Protein Information

SUMO-activating enzyme subunit 1

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

UniProt Comments for SAE1

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle

Research Articles on SAE1

1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SAE1 antibody

Reference Product	PubMed Publications
SUMO1 antibody	>32 publications with SAE1 and SUMO1
UBA2 antibody	>25 publications with SAE1 and UBA2
SUMO2 antibody	>5 publications with SAE1 and SUMO2
SUMO3 antibody	>4 publications with SAE1 and SUMO3
UBE2I antibody	>3 publications with SAE1 and UBE2I
RBX1 antibody	>1 publications with SAE1 and RBX1
UBC antibody	>1 publications with SAE1 and UBC

Pathways associated with anti-SAE1 antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Processing And Activation Of SUMO Pathway antibodies	Processing And Activation Of SUMO Pathway Diagram
SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway antibodies	SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway Diagram
SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway antibodies	SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway Diagram
SUMOylation Pathway antibodies	SUMOylation Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-SAE1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>4 publications with SAE1 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>4 publications with SAE1 and Neoplasms, Experimental
Mental Disorders Antibodies	>3 publications with SAE1 and Mental Disorders
Disease Models, Animal Antibodies	>3 publications with SAE1 and Disease Models, Animal
Brain Diseases Antibodies	>2 publications with SAE1 and Brain Diseases
Abnormalities, Multiple Antibodies	>1 publications with SAE1 and Abnormalities, Multiple
Muscular Diseases Antibodies	>1 publications with SAE1 and Muscular Diseases
Seizures Antibodies	>1 publications with SAE1 and Seizures

Organs/Tissues associated with anti-SAE1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with SAE1 and Brain
Muscle Antibodies	>2 publications with SAE1 and Muscle
Blood Antibodies	>2 publications with SAE1 and Blood
Skin Antibodies	>2 publications with SAE1 and Skin
Thyroid Antibodies	>1 publications with SAE1 and Thyroid
Connective Tissue Antibodies	>1 publications with SAE1 and Connective Tissue
Kidney Antibodies	>1 publications with SAE1 and Kidney
Uterus Antibodies	>1 publications with SAE1 and Uterus
Embryonic Tissue Antibodies	>1 publications with SAE1 and Embryonic Tissue
Stomach Antibodies	>1 publications with SAE1 and Stomach