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SSP29/ANP32B (ANP32B) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421267

MBS421267 is an antibody targeting ANP32B. Also known as Goat anti-SSP29/ANP32B Antibody. Reactive in Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SSP29/ANP32B, Polyclonal Antibody
Full Product Name
Goat anti-SSP29/ANP32B Antibody
Product Gene Name
anti-ANP32B antibody
Product Synonym Gene Name
SSP29
Product Synonym Names
Goat Anti-SSP29 / ANP32B, Biotinylated Antibody; ANP32B; SSP29; APRIL; PHAPI2; acidic (leucine-rich) nuclear phosphoprotein 32 family, member B; acidic protein rich in leucines; OTTHUMP00000063770; ANP32B antibody; SSP29 antibody; APRIL antibody; PHAPI2 antibody; acidic (leucine-rich) nuclear phosphoprotein 32 family; member B antibody; acidic protein rich in leucines antibody; OTTHUMP00000063770 antibody; SSP29 / ANP32B
Immunogen Sequence
KRKRETDDEG EDD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Matching Pairs
Unconjugated Antibody: SSP29/ANP32B (MBS421267)
Biotin Conjugated Antibody: SSP29/ANP32B (MBS423521)
Antibody/Peptide Pairs
SSP29 / ANP32B peptide (MBS426371) is used for blocking the activity of SSP29/ANP32B antibody (MBS421267)
Molecular Weight
22,277 Da
Immunogen
Peptide with sequence C-KRKRETDDEGEDD, from the C Terminus of the protein sequence according to NP_006392.1.
Epitope
C Terminus
Note
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ANP32B antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ANP32B antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in lobular cells. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 28kDa band observed in Human Tonsil lysates, and approx 30kDa band observed in Rat and Pig Spleen lysates and in lysates of cell line NIH3T3 (calculated MW of 28.8kDa according to Human NP_006392.1, 31.1kDa according to Mouse NP_570959.1 and to Rat NP_571986.1 and 29.9kDa according to Pig XP_013849138.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-ANP32B antibody

(0.1ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ANP32B Polyclonal Antibody – Cat# MBS421267

Western Blot (WB) of anti-ANP32B antibody

(0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ANP32B Polyclonal Antibody, Western Blot (WB) – Cat# MBS421267

Immunohistochemistry (IHC) of anti-ANP32B antibody

(3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-ANP32B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421267

Other Notes

Small volumes of anti-ANP32B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ANP32B. It may not necessarily be applicable to this product.

NCBI GI #
5454088
NCBI GeneID
10541
NCBI Accession #
NP_006392.1
UniProt Secondary Accession #
O00655; P78458; P78459; B2R9C7
UniProt Related Accession #
Q92688
GenBank Nucleotide #
NM_006401.2
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member B
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member B
NCBI Official Symbol
ANP32B
NCBI Official Synonym Symbols
APRIL; SSP29; PHAPI2
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member B
UniProt Synonym Protein Names
Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2; PHAPI2; Silver-stainable protein SSP29
Protein Family
Acidic leucine-rich nuclear phosphoprotein 32 family
UniProt Gene Name
ANP32B
UniProt Synonym Gene Names
APRIL; PHAPI2; PHAPI2
UniProt Entry Name
AN32B_HUMAN
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member B

UniProt Comments for ANP32B

ANP32B: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Belongs to the ANP32 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 9q22.32

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: histone binding; protein binding

Biological Process: caspase activation; inner ear development; negative regulation of cell differentiation; nucleosome assembly; palate development; positive regulation of protein export from nucleus; vasculature development; ventricular system development
Show more

Research Articles on ANP32B

1. Caspase-3-resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANP32B antibody

 Reference Product  PubMed Publications
 ELAVL1 antibody  >8 publications with ANP32B and ELAVL1
 KLF5 antibody  >1 publications with ANP32B and KLF5

Diseases associated with anti-ANP32B antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >29 publications with ANP32B and Necrosis
 Inflammation Antibodies  >7 publications with ANP32B and Inflammation
 Nervous System Diseases Antibodies  >4 publications with ANP32B and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with ANP32B and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >1 publications with ANP32B and Breast Neoplasms
 Urinary Bladder Neoplasms Antibodies  >1 publications with ANP32B and Urinary Bladder Neoplasms
 Liver Neoplasms Antibodies  >1 publications with ANP32B and Liver Neoplasms
 Disease Models, Animal Antibodies  >1 publications with ANP32B and Disease Models, Animal
 Heart Diseases Antibodies  >1 publications with ANP32B and Heart Diseases
 Hyperplasia Antibodies  >1 publications with ANP32B and Hyperplasia

Organs/Tissues associated with anti-ANP32B antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with ANP32B and Blood
 Nerve Antibodies  >6 publications with ANP32B and Nerve
 Brain Antibodies  >5 publications with ANP32B and Brain
 Spleen Antibodies  >3 publications with ANP32B and Spleen
 Bone Antibodies  >2 publications with ANP32B and Bone
 Bone Marrow Antibodies  >2 publications with ANP32B and Bone Marrow
 Embryonic Tissue Antibodies  >2 publications with ANP32B and Embryonic Tissue
 Heart Antibodies  >1 publications with ANP32B and Heart
 Muscle Antibodies  >1 publications with ANP32B and Muscle
 Prostate Antibodies  >1 publications with ANP32B and Prostate
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