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OSBPL5 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421287

MBS421287 is an antibody targeting OSBPL5. Also known as Goat anti-ORP5 (OBPH1) Antibody. Reactive in Tested: Human;Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ORP5 (OSBPL5), Polyclonal Antibody
Full Product Name
Goat anti-ORP5 (OBPH1) Antibody
Product Gene Name
Product Synonym Gene Name
ORP5; OBPH1
Product Synonym Names
OSBPL5; ORP5; OBPH1; FLJ31948; KIAA1534; DKFZp586H1719; oxysterol binding protein-like 5; OSBP-related protein 5; oxysterol-binding protein homologue 1; oxysterol-binding protein-related protein 5; FLJ42929; oxysterol-binding protein-like protein 5; OSBPL5 antibody; ORP5 antibody; OBPH1 antibody; FLJ31948 antibody; KIAA1534 antibody; DKFZp586H1719 antibody; oxysterol binding protein-like 5 antibody; OSBP-related protein 5 antibody; oxysterol-binding protein homologue 1 antibody; oxysterol-binding protein-related protein 5 antibody; FLJ42929 antibody; oxysterol-binding protein-like protein 5 antibody; ORP5 (OBPH1)
Immunogen Sequence
KEEAFLRRRF SLC
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human;
Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ORP5 peptide (MBS425184) is used for blocking the activity of ORP5 antibody (MBS421287)
OMIM
606733
Immunogen
Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1; NP_663613.1; NP_001137535.1.
Epitope
N Terminus
Note
This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-OSBPL5 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-OSBPL5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 110kDa band observed in human brain (cerebellum) and spleen lysates (calculated MW of 98.4kDa according to NP_065947.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 ug/ml.

Western Blot (WB) of anti-OSBPL5 antibody

(2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-OSBPL5 Polyclonal Antibody – Cat# MBS421287

Immunohistochemistry (IHC) of anti-OSBPL5 antibody

MBS421287 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-OSBPL5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421287

Immunohistochemistry (IHC) of anti-OSBPL5 antibody

MBS421287 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-OSBPL5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421287

Other Notes

Small volumes of anti-OSBPL5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OSBPL5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
oxysterol-binding protein-related protein 5 isoform a
NCBI Official Synonym Full Names
oxysterol binding protein like 5
NCBI Official Symbol
OSBPL5
NCBI Official Synonym Symbols
ORP5; OBPH1
UniProt Protein Name
Oxysterol-binding protein-related protein 5
UniProt Synonym Protein Names
Oxysterol-binding protein homolog 1
UniProt Gene Name
OSBPL5
UniProt Synonym Gene Names
; ORP-5; OSBP-related protein 5
UniProt Entry Name
OSBL5_HUMAN
NCBI Protein Information
oxysterol-binding protein-related protein 5

NCBI Summary for OSBPL5

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that play a key role in the maintenance of cholesterol balance in the body. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. This gene has been shown to be imprinted, with preferential expression from the maternal allele only in placenta. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
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UniProt Comments for OSBPL5

OSBPL5: May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Belongs to the OSBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytosol; endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: cholesterol binding; oxysterol binding; phosphatidylserine binding; phospholipid transporter activity

Biological Process: cholesterol metabolic process; cholesterol transport; Golgi to plasma membrane transport; phospholipid transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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