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DUSP1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421313

MBS421313 is an antibody targeting DUSP1. Also known as Goat anti-DUSP1 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
DUSP1, Polyclonal Antibody
Full Product Name
Goat anti-DUSP1 Antibody
Product Gene Name
anti-DUSP1 antibody
Product Synonym Names
DUSP1; dual specificity phosphatase 1; HVH1; CL100; MKP-1; PTPN10; serine/threonine specific protein phosphatase; MKP1; DUSP1 antibody; dual specificity phosphatase 1 antibody; HVH1 antibody; CL100 antibody; MKP-1 antibody; PTPN10 antibody; serine/threonine specific protein phosphatase antibody; MKP1 antibody
Immunogen Sequence
SYLQSPITTS PSC
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
DUSP1 peptide (MBS426138) is used for blocking the activity of DUSP1 antibody (MBS421313)
Molecular Weight
39,298 Da
OMIM
600714
Immunogen
Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-DUSP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DUSP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration, 3-5ug/ml.
Western Blot: approx. 40kDa band observed in Human lysates of HeLa (calculated MW of 39.3kDa according to NP_004408.1). Recommended concentration: 0.3-1ug/ml. An anonymous customer found positive results in WB on Mouse microglia (BV2 cells). An anonymous customer found positive results in WB on Rat primary microglia. An anonymous customer found positive results in WB on Rat spinal cord homogenate.

Western Blot (WB) of anti-DUSP1 antibody

(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DUSP1 Polyclonal Antibody – Cat# MBS421313

Immunohistochemistry (IHC) of anti-DUSP1 antibody

(3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-DUSP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421313

Other Notes

Small volumes of anti-DUSP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DUSP1. It may not necessarily be applicable to this product.

NCBI GI #
4758204
NCBI GeneID
1843
NCBI Accession #
NP_004408.1
UniProt Secondary Accession #
Q2V508; D3DQL9
UniProt Related Accession #
P28562
GenBank Nucleotide #
NM_004417.3
NCBI Official Full Name
dual specificity protein phosphatase 1
NCBI Official Synonym Full Names
dual specificity phosphatase 1
NCBI Official Symbol
DUSP1
NCBI Official Synonym Symbols
HVH1; MKP1; CL100; MKP-1; PTPN10
UniProt Protein Name
Dual specificity protein phosphatase 1
UniProt Synonym Protein Names
Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100
Protein Family
Dual specificity protein phosphatase
UniProt Gene Name
DUSP1
UniProt Synonym Gene Names
CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1
UniProt Entry Name
DUS1_HUMAN
NCBI Protein Information
dual specificity protein phosphatase 1

NCBI Summary for DUSP1

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]
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UniProt Comments for DUSP1

MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus

Molecular Function: growth factor binding; MAP kinase tyrosine/serine/threonine phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity; protein binding; protein tyrosine/threonine phosphatase activity

Biological Process: cellular response to hormone stimulus; endoderm formation; inactivation of MAPK activity; negative regulation of apoptosis; negative regulation of MAP kinase activity; negative regulation of MAPKKK cascade; negative regulation of meiotic cell cycle; positive regulation of apoptosis; regulation of apoptosis; response to calcium ion; response to cAMP; response to estradiol stimulus; response to glucocorticoid stimulus; response to hydrogen peroxide; response to light stimulus; response to oxidative stress; response to retinoic acid; response to testosterone stimulus
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Research Articles on DUSP1

1. MKP-1 is a MAPK deactivator; thus, by controlling p38 MAPK phosphorylation status in a temporally distinct manner, MKP-1 ensures that TTP is expressed and made functional at precisely the correct time to repress cytokine expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DUSP1 antibody

 Reference Product  PubMed Publications
 JUN antibody  >231 publications with DUSP1 and JUN
 ATF2 antibody  >10 publications with DUSP1 and ATF2
 MAPK14 antibody  >8 publications with DUSP1 and MAPK14
 MAPK8 antibody  >6 publications with DUSP1 and MAPK8
 TP53 antibody  >4 publications with DUSP1 and TP53
 MAPK3 antibody  >4 publications with DUSP1 and MAPK3
 UBC antibody  >1 publications with DUSP1 and UBC
 MAPK12 antibody  >1 publications with DUSP1 and MAPK12

Pathways associated with anti-DUSP1 antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR1 Pathway antibodies  Downstream Signaling Of Activated FGFR1 Pathway Diagram

Diseases associated with anti-DUSP1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >134 publications with DUSP1 and Inflammation
 Necrosis Antibodies  >104 publications with DUSP1 and Necrosis
 Lung Diseases Antibodies  >56 publications with DUSP1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >46 publications with DUSP1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >37 publications with DUSP1 and Nervous System Diseases
 Liver Diseases Antibodies  >36 publications with DUSP1 and Liver Diseases
 Breast Neoplasms Antibodies  >34 publications with DUSP1 and Breast Neoplasms
 Hypertension Antibodies  >27 publications with DUSP1 and Hypertension
 Neoplasms, Experimental Antibodies  >26 publications with DUSP1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >24 publications with DUSP1 and Liver Neoplasms

Organs/Tissues associated with anti-DUSP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >113 publications with DUSP1 and Blood
 Muscle Antibodies  >94 publications with DUSP1 and Muscle
 Brain Antibodies  >83 publications with DUSP1 and Brain
 Vascular Antibodies  >80 publications with DUSP1 and Vascular
 Lung Antibodies  >78 publications with DUSP1 and Lung
 Liver Antibodies  >58 publications with DUSP1 and Liver
 Bone Antibodies  >51 publications with DUSP1 and Bone
 Heart Antibodies  >47 publications with DUSP1 and Heart
 Kidney Antibodies  >32 publications with DUSP1 and Kidney
 Nerve Antibodies  >30 publications with DUSP1 and Nerve
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