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MPG Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421319

MBS421319 is an antibody targeting MPG. Also known as Goat anti-MPG Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MPG, Polyclonal Antibody
Full Product Name
Goat anti-MPG Antibody
Product Gene Name
Product Synonym Names
MDG; N-methylpurine-DNA glycosylase; AAG; APNG; CRA36.1; Mid1; N-methylpurine-DNA glycosylase, MPG; PIG11; PIG16; anpg; 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; CRA36.1 (3-methyl-adenine DNA glycosylase); alkylad; N-methylpurine-DNA glycosylase antibody; AAG antibody; APNG antibody; CRA36.1 antibody; MDG antibody; Mid1 antibody; N-methylpurine-DNA glycosylase; MPG antibody; PIG11 antibody; PIG16 antibody; anpg antibody; 3\1 end of the Mid1 gene; localized 68 kb upstream the humanzeta globin gene on 16p antibody; CRA36.1 (3-methyl-adenine DNA glycosylase) antibody; alkyladenine DNA glycosylase antibody; proliferation-inducing protein 11 antibody; proliferation-inducing protein 16 antibody
Immunogen Sequence
SVVDRVAEQD TQA
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MPG peptide (MBS425627) is used for blocking the activity of MPG antibody (MBS421319)
Molecular Weight
30,824 Da
OMIM
156565
Immunogen
Peptide with sequence C-SVVDRVAEQDTQA, from the C Terminus of the protein sequence according to NP_002425.2; NP_001015052.1; NP_001015054.1.
Epitope
C Terminus
Note
This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MPG antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MPG antibody
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-MPG antibody

Staining (1ug/ml) of HEK293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-MPG Polyclonal Antibody – Cat# MBS421319

Other Notes

Small volumes of anti-MPG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MPG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-3-methyladenine glycosylase isoform a
NCBI Official Synonym Full Names
N-methylpurine DNA glycosylase
NCBI Official Symbol
MPG
NCBI Official Synonym Symbols
AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1
UniProt Protein Name
DNA-3-methyladenine glycosylase
UniProt Synonym Protein Names
3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase
UniProt Gene Name
MPG
UniProt Synonym Gene Names
AAG; ANPG; MID1
UniProt Entry Name
3MG_HUMAN
NCBI Protein Information
DNA-3-methyladenine glycosylase

UniProt Comments for MPG

MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.2.2.21; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm

Molecular Function: alkylbase DNA N-glycosylase activity; damaged DNA binding; DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; protein binding

Biological Process: base-excision repair; depurination; DNA dealkylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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