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DDAH2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421337

MBS421337 is an antibody targeting DDAH2. Also known as Goat anti-DDAH2 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DDAH2, Polyclonal Antibody
Full Product Name
Goat anti-DDAH2 Antibody
Product Gene Name
Product Synonym Names
DDAH2; dimethylarginine dimethylaminohydrolase 2; DDAH; G6a; NG30; DDAHII; dimethylarginine dimethylaminohydrolase II; NG-dimethylarginine dimethylamino hydrolase homolog; OTTHUMP00000062666; DADB-110M10.5; OTTHUMP00000029307; OTTHUMP00000174406; alternat; DDAH2 antibody; dimethylarginine dimethylaminohydrolase 2 antibody; DDAH antibody; G6a antibody; NG30 antibody; DDAHII antibody; dimethylarginine dimethylaminohydrolase II antibody; NG-dimethylarginine dimethylamino hydrolase homolog antibody; OTTHUMP00000062666 antibody; DADB-110M10.5 antibody; OTTHUMP00000029307 antibody; OTTHUMP00000174406 antibody; alternative name: NG30; G6a antibody; dimethylarginine dimethylaminohydrolase II antibody
Immunogen Sequence
SSLCLVLSTR PHS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
DDAH2 peptide (MBS426690) is used for blocking the activity of DDAH2 antibody (MBS421337)
Molecular Weight
29,644 Da
OMIM
604744
Immunogen
Peptide with sequence SSLCLVLSTRPHS, from the C Terminus of the protein sequence according to NP_039268.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-DDAH2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-DDAH2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-DDAH2 antibody

(0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DDAH2 Polyclonal Antibody – Cat# MBS421337

Other Notes

Small volumes of anti-DDAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DDAH2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
NCBI Official Synonym Full Names
dimethylarginine dimethylaminohydrolase 2
NCBI Official Symbol
DDAH2
NCBI Official Synonym Symbols
G6a; DDAH; NG30; DDAHII; HEL-S-277
UniProt Protein Name
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
UniProt Synonym Protein Names
DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein
UniProt Gene Name
DDAH2
UniProt Synonym Gene Names
DDAH; G6A; NG30; DDAH-2; Dimethylarginine dimethylaminohydrolase 2
UniProt Entry Name
DDAH2_HUMAN
NCBI Protein Information
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

NCBI Summary for DDAH2

This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants. [provided by RefSeq, Dec 2014]
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UniProt Comments for DDAH2

DDAH2: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family.

Protein type: EC 3.5.3.18; Apoptosis; Hydrolase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: centrosome; cytoplasm; cytosol; mitochondrion

Molecular Function: amino acid binding; catalytic activity; dimethylargininase activity; protein binding

Biological Process: arginine catabolic process; citrulline metabolic process; negative regulation of apoptosis; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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