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PTBP2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421401

MBS421401 is an antibody targeting PTBP2. Also known as Goat anti-PTBP2 Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PTBP2, Polyclonal Antibody
Full Product Name
Goat anti-PTBP2 Antibody
Product Gene Name
Product Synonym Names
PTBP2; polypyrimidine tract binding protein 2; FLJ34897; MIBP; PTB; PTBLP; brPTB; nPTB; nPTB5; nPTB6; nPTB7; nPTB8; PTB-like; neural polypyrimidine tract binding protein; splicing regulator; PTBP2 antibody; polypyrimidine tract binding protein 2 antibody; FLJ34897 antibody; MIBP antibody; PTB antibody; PTBLP antibody; brPTB antibody; nPTB antibody; nPTB5 antibody; nPTB6 antibody; nPTB7 antibody; nPTB8 antibody; PTB-like antibody; neural polypyrimidine tract binding protein antibody; splicing regulator antibody
Immunogen Sequence
SKKFKGEDKM D
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PTBP2 peptide (MBS426620) is used for blocking the activity of PTBP2 antibody (MBS421401)
Molecular Weight
38,336 Da
OMIM
608449
Immunogen
Peptide with sequence C-SKKFKGEDKMD, from the internal region of the protein sequence according to NP_067013.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PTBP2 antibody
Peptide ELISA (EIA)
Application Notes for anti-PTBP2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Preliminary experiments gave an approx 35kDa band in lysates of cell line NIH3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_067013.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-PTBP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTBP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
polypyrimidine tract-binding protein 2 isoform 6
NCBI Official Synonym Full Names
polypyrimidine tract binding protein 2
NCBI Official Symbol
PTBP2
NCBI Official Synonym Symbols
nPTB; PTBLP; brPTB
UniProt Protein Name
Polypyrimidine tract-binding protein 2
UniProt Synonym Protein Names
Neural polypyrimidine tract-binding protein; Neurally-enriched homolog of PTB; PTB-like protein
UniProt Gene Name
PTBP2
UniProt Synonym Gene Names
NPTB; PTB; PTBLP
UniProt Entry Name
PTBP2_HUMAN
NCBI Protein Information
polypyrimidine tract-binding protein 2

NCBI Summary for PTBP2

The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
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UniProt Comments for PTBP2

PTBP2: RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre- mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cell soma; growth cone; spliceosome

Molecular Function: mRNA binding; nucleotide binding

Biological Process: cerebellum development; mRNA splice site selection; negative regulation of RNA splicing; spinal cord development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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