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PGAM1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421435

MBS421435 is an antibody targeting PGAM1. Also known as Goat anti-PGAM1/PGAM2/PGAM4 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PGAM1/PGAM2/PGAM4, Polyclonal Antibody
Full Product Name
Goat anti-PGAM1/PGAM2/PGAM4 Antibody
Product Gene Name
anti-PGAM1 antibody
Product Synonym Gene Name
PGAM2; PGAM4
Product Synonym Names
PGAM1; PGAM2; phosphoglycerate mutase 1 (brain); PGAMA; RP11-452K12.8; OTTHUMP00000059414; Phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase 2 (muscle); MGC88743; PGAMM; Phosphoglycerate mutase, muscle form; PGAM3; phosphoglycerate mutas; PGAM1 antibody; PGAM2 antibody; phosphoglycerate mutase 1 (brain) antibody; PGAMA antibody; RP11-452K12.8 antibody; OTTHUMP00000059414 antibody; Phosphoglycerate mutase A; nonmuscle form antibody; phosphoglycerate mutase 2 (muscle) antibody; MGC88743 antibody; PGAMM antibody; Phosphoglycerate mutase; muscle form antibody; PGAM3 antibody; phosphoglycerate mutase family member 4 antibody; PGAM-B antibody; dJ1000K24.1 antibody; phosphoglycerate mutase family 3 antibody; phosphoglycerate mutase family 4 antibody; phosphoglycerate mutase processed protein antibody; bisphosphoglycerate mutase 1 antibody; bisphosphoglycerate mutase 2 antibody; bisphosphoglycerate mutase 4 antibody; BPG-dependent PGAM 1 antibody; OTTHUMP00000020190 antibody; PGAM-M antibody; BPG-dependent PGAM 2 antibody; OTTHUMP00000063546 antibody; PGAM1 / PGAM2 / PGAM4
Immunogen Sequence
KAMEAVAAQG KAKK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PGAM1 / PGAM2 / PGAM4 peptide (MBS425993) is used for blocking the activity of PGAM1/PGAM2/PGAM4 antibody (MBS421435)
Molecular Weight
28,804 Da
OMIM
172250
Immunogen
Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1.
Epitope
C Terminus
Note
Please note this antibody is expected to recognize the products of 3 highly similar genes.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PGAM1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PGAM1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 27kDa band observed in Human Liver and Human Brain lysates (calculated MW of 28.8kDa according to NP_002620.1, NP_000281.2, NP_001025062.1). Recommended concentration: 0.01-0.03ug/ml.

Western Blot (WB) of anti-PGAM1 antibody

Staining (0. 01ug/ml) of human liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-PGAM1 Polyclonal Antibody – Cat# MBS421435

Other Notes

Small volumes of anti-PGAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM1. It may not necessarily be applicable to this product.

NCBI GI #
4505753
NCBI GeneID
5223
NCBI Accession #
NP_002620.1
NCBI Related Accession #
NP_000281.2; NP_001025062.1
UniProt Secondary Accession #
Q9BWC0
UniProt Related Accession #
P18669
GenBank Nucleotide #
NM_002629.3
NCBI Official Full Name
phosphoglycerate mutase 1 isoform 1
NCBI Official Synonym Full Names
phosphoglycerate mutase 1
NCBI Official Symbol
PGAM1
NCBI Official Synonym Symbols
PGAMA; PGAM-B; HEL-S-35
UniProt Protein Name
Phosphoglycerate mutase 1
UniProt Synonym Protein Names
BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B
Protein Family
Phosphoglycerate mutase
UniProt Gene Name
PGAM1
UniProt Synonym Gene Names
PGAMA; PGAM-B
UniProt Entry Name
PGAM1_HUMAN
NCBI Protein Information
phosphoglycerate mutase 1

NCBI Summary for PGAM1

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

UniProt Comments for PGAM1

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.4; EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Phosphatase (non-protein); EC 5.4.2.11

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: cytoplasm; cytosol; membrane; myelin sheath

Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; phosphoglycerate mutase activity; protein binding; protein kinase binding

Biological Process: dephosphorylation; gluconeogenesis; glycolysis; regulation of glycolysis; regulation of pentose-phosphate shunt; respiratory burst
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Research Articles on PGAM1

1. PGAM is acetylated at lysines 100/106/113/138 in its central region, and a member of the Sirtuin family (class III deacetylase), SIRT2, is responsible for its deacetylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PGAM1 antibody

 Reference Product  PubMed Publications
 GAPDH antibody  >5 publications with PGAM1 and GAPDH
 PGK1 antibody  >4 publications with PGAM1 and PGK1
 ALDOC antibody  >2 publications with PGAM1 and ALDOC
 ALDOA antibody  >2 publications with PGAM1 and ALDOA
 UBC antibody  >1 publications with PGAM1 and UBC

Pathways associated with anti-PGAM1 antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with anti-PGAM1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >5 publications with PGAM1 and Carcinoma
 Lung Neoplasms Antibodies  >2 publications with PGAM1 and Lung Neoplasms
 Liver Neoplasms Antibodies  >1 publications with PGAM1 and Liver Neoplasms
 Heart Diseases Antibodies  >1 publications with PGAM1 and Heart Diseases
 Hyperplasia Antibodies  >1 publications with PGAM1 and Hyperplasia
 Fatty Liver Antibodies  >1 publications with PGAM1 and Fatty Liver
 Necrosis Antibodies  >1 publications with PGAM1 and Necrosis
 Mammary Neoplasms, Animal Antibodies  >1 publications with PGAM1 and Mammary Neoplasms, Animal
 Carcinoma, Non-Small-Cell Lung Antibodies  >1 publications with PGAM1 and Carcinoma, Non-Small-Cell Lung
 Carcinoma, Squamous Cell Antibodies  >1 publications with PGAM1 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-PGAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with PGAM1 and Brain
 Blood Antibodies  >8 publications with PGAM1 and Blood
 Testis Antibodies  >4 publications with PGAM1 and Testis
 Muscle Antibodies  >4 publications with PGAM1 and Muscle
 Liver Antibodies  >4 publications with PGAM1 and Liver
 Kidney Antibodies  >3 publications with PGAM1 and Kidney
 Lung Antibodies  >3 publications with PGAM1 and Lung
 Heart Antibodies  >3 publications with PGAM1 and Heart
 Ovary Antibodies  >2 publications with PGAM1 and Ovary
 Nerve Antibodies  >2 publications with PGAM1 and Nerve
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