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AIF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421481

MBS421481 is an antibody targeting AIF1. Also known as Goat anti-AIF1/IBA1 (isoform 3) Antibody. Reactive in Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AIF1/IBA1, Polyclonal Antibody
Full Product Name
Goat anti-AIF1/IBA1 (isoform 3) Antibody
Product Gene Name
anti-AIF1 antibody
Product Synonym Gene Name
AIF1; IBA1
Product Synonym Names
Goat Anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody; AIF1; IBA1; allograft inflammatory factor 1; DASS-82G15.2; AIF-1; IRT-1; Em: AF129756.17; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; AIF1 antibody; IBA1 antibody; allograft inflammatory factor 1 antibody; DASS-82G15.2 antibody; AIF-1 antibody; IRT-1 antibody; Em: AF129756.17 antibody; interferon gamma responsive transcript antibody; ionized calcium-binding adapter molecule antibody; AIF1 / IBA1 (isoform 3)
Immunogen Sequence
NKQFLDDPKY SSDED
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
AIF1 / IBA1 peptide (MBS428384) is used for blocking the activity of AIF1/IBA1 antibody (MBS421481)
OMIM
601833
Immunogen
Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.
Note
This antibody is expected to recognise isoform 3 (NP_001614.3) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-AIF1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC)
Application Notes for anti-AIF1 antibody
ELISA: Antibody detection limit dilution 1:128000.
IHC: In paraffin embedded human lung. Recommended concentration: 6µg/ml.
FC: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
IF: Strong expression of the protein seen in the cytoplasm of U937 and CaCo-2 cells. Recommended concentration: 10µg/ml.

Western Blot (WB) of anti-AIF1 antibody

(0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-AIF1 Polyclonal Antibody – Cat# MBS421481

Immunohistochemistry (IHC) of anti-AIF1 antibody

(6µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-AIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421481

Immunohistochemistry (IHC) of anti-AIF1 antibody

Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.
Goat Anti-AIF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421481

Flow Cytometry (FC) of anti-AIF1 antibody

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat Anti-AIF1 Polyclonal Antibody, Flow Cytometry (FC) – Cat# MBS421481

Immunofluorescence (IF) of anti-AIF1 antibody

MBS421481 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-AIF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421481

Immunofluorescence (IF) of anti-AIF1 antibody

Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-AIF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421481

Immunofluorescence (IF) of anti-AIF1 antibody

Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-AIF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421481

Other Notes

Small volumes of anti-AIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AIF1. It may not necessarily be applicable to this product.

NCBI GI #
14574568
NCBI GeneID
199
NCBI Accession #
NP_001614.3
UniProt Secondary Accession #
O43904; Q9UIV4; Q9UKS9; A8K406
UniProt Related Accession #
P55008
GenBank Nucleotide #
NM_001623.4
NCBI Official Full Name
allograft inflammatory factor 1 isoform 3
NCBI Official Synonym Full Names
allograft inflammatory factor 1
NCBI Official Symbol
AIF1
NCBI Official Synonym Symbols
IBA1; IRT1; AIF-1; IRT-1
UniProt Protein Name
Allograft inflammatory factor 1
UniProt Synonym Protein Names
Ionized calcium-binding adapter molecule 1; Protein G1
Protein Family
Allograft inflammatory factor
UniProt Gene Name
AIF1
UniProt Synonym Gene Names
G1; IBA1; AIF-1
UniProt Entry Name
AIF1_HUMAN
NCBI Protein Information
allograft inflammatory factor 1

NCBI Summary for AIF1

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
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UniProt Comments for AIF1

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: actin filament; cytoplasm; cytosol; lamellipodium; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; actin filament polymerization; cellular response to extracellular stimulus; cellular response to hormone stimulus; inflammatory response; microglial cell activation; negative regulation of apoptosis; negative regulation of smooth muscle cell proliferation; phagocytosis, engulfment; positive regulation of muscle hyperplasia; positive regulation of nitric oxide biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; Rac protein signal transduction; response to axon injury; response to electrical stimulus; response to glucocorticoid stimulus
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Research Articles on AIF1

1. Data showed that AIF-1 promoted the proliferation of HepG2 cells by accelerating the activation of IGF-1R and its downstream signaling pathway, which confirms that AIF-1 plays a crucial role in the development of hepatocellular carcinoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AIF1 antibody

 Reference Product  PubMed Publications
 GFAP antibody  >142 publications with AIF1 and GFAP
 CD68 antibody  >65 publications with AIF1 and CD68
 OLIG2 antibody  >13 publications with AIF1 and OLIG2
 DCX antibody  >8 publications with AIF1 and DCX
 MBP antibody  >7 publications with AIF1 and MBP
 TSPO antibody  >5 publications with AIF1 and TSPO
 ITGAM antibody  >5 publications with AIF1 and ITGAM
 RBFOX3 antibody  >2 publications with AIF1 and RBFOX3
 BAG6 antibody  >1 publications with AIF1 and BAG6

Pathways associated with anti-AIF1 antibody

 Products by Pathway  Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-AIF1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >358 publications with AIF1 and Disease Models, Animal
 Inflammation Antibodies  >204 publications with AIF1 and Inflammation
 Cardiovascular Diseases Antibodies  >127 publications with AIF1 and Cardiovascular Diseases
 Necrosis Antibodies  >111 publications with AIF1 and Necrosis
 Nerve Degeneration Antibodies  >54 publications with AIF1 and Nerve Degeneration
 Poisoning Antibodies  >21 publications with AIF1 and Poisoning
 Neurotoxicity Syndromes Antibodies  >19 publications with AIF1 and Neurotoxicity Syndromes
 Learning Disorders Antibodies  >19 publications with AIF1 and Learning Disorders
 Cognition Disorders Antibodies  >18 publications with AIF1 and Cognition Disorders
 Parkinson Disease Antibodies  >18 publications with AIF1 and Parkinson Disease

Organs/Tissues associated with anti-AIF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >534 publications with AIF1 and Brain
 Blood Antibodies  >178 publications with AIF1 and Blood
 Vascular Antibodies  >73 publications with AIF1 and Vascular
 Muscle Antibodies  >53 publications with AIF1 and Muscle
 Bone Marrow Antibodies  >45 publications with AIF1 and Bone Marrow
 Eye Antibodies  >38 publications with AIF1 and Eye
 Embryonic Tissue Antibodies  >25 publications with AIF1 and Embryonic Tissue
 Heart Antibodies  >24 publications with AIF1 and Heart
 Liver Antibodies  >19 publications with AIF1 and Liver
 Spleen Antibodies  >17 publications with AIF1 and Spleen
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