Goat Anti-NET1 Polyclonal Antibody – Cat# MBS421507

(2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

NET1 Antibody – Goat Polyclonal

Name: NET1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421507
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421507

MBS421507 is an antibody targeting NET1. Also known as Goat anti-NET1/ARHGEF8 (Internal) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NET1/ARHGEF8, Polyclonal Antibody

Full Product Name

Goat anti-NET1/ARHGEF8 (Internal) Antibody

Product Gene Name

anti-NET1 antibody

Product Synonym Gene Name

ARHGEF8

Product Synonym Names

Goat Anti-NET1 / ARHGEF8 (C Terminus) Antibody; NET1; ARHGEF8; neuroepithelial cell transforming gene 1; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding prot; NET1 antibody; ARHGEF8 antibody; neuroepithelial cell transforming gene 1 antibody; NET1A antibody; Rho guanine nucleotide exchange factor (GEF) 8 antibody; guanine nucleotide regulatory protein (oncogene) antibody; neuroepithelioma transforming gene 1 antibody; p65 Net1 proto-oncogene protein antibody; small GTP-binding protein regulator antibody; NET1 / ARHGEF8 (Internal)

Immunogen Sequence

DKLEYLDEKQ RDPR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

NET1 / ARHGEF8 peptide (MBS426865) is used for blocking the activity of NET1/ARHGEF8 antibody (MBS421507)

Molecular Weight

61,885 Da

OMIM

606450

Immunogen

Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2; NP_001040625.1.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-NET1 antibody

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-NET1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.
Western Blot: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NET1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NET1. It may not necessarily be applicable to this product.

NCBI GI #

19923327

NCBI GeneID

10276

NCBI Accession #

NP_005854.2

NCBI Related Accession #

NP_001040625.1

UniProt Secondary Accession #

Q12773; Q96D82; Q99903; Q9UEN6

UniProt Related Accession #

Q7Z628

GenBank Nucleotide #

NM_005863.4

NCBI Official Full Name

neuroepithelial cell-transforming gene 1 protein isoform 2

NCBI Official Synonym Full Names

neuroepithelial cell transforming 1

NCBI Official Symbol

NET1

NCBI Official Synonym Symbols

NET1A; ARHGEF8

UniProt Protein Name

Neuroepithelial cell-transforming gene 1 protein

UniProt Synonym Protein Names

Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8

Protein Family

Neuroepithelial cell-transforming gene 1 protein

UniProt Gene Name

NET1

UniProt Synonym Gene Names

ARHGEF8

UniProt Entry Name

ARHG8_HUMAN

NCBI Protein Information

neuroepithelial cell-transforming gene 1 protein

NCBI Summary for NET1

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

UniProt Comments for NET1

NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; GEFs, Rac/Rho; Motility/polarity/chemotaxis; GEFs

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: cytosol; nucleus; plasma membrane

Molecular Function: GTP-Rho binding; guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: myoblast migration; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of cell growth; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; signal transduction

Research Articles on NET1

1. Simultaneous silencing of NET-1 and survivin using one-chain-double-target siRNA thus provides an advantageous alternative in the development of therapeutics for skin squamous cell carcinoma .

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NET1 antibody

Reference Product	PubMed Publications
RHOA antibody	>27 publications with NET1 and RHOA
CDC42 antibody	>5 publications with NET1 and CDC42
RAC1 antibody	>5 publications with NET1 and RAC1
RHOB antibody	>4 publications with NET1 and RHOB
RHOU antibody	>2 publications with NET1 and RHOU
ARHGAP6 antibody	>1 publications with NET1 and ARHGAP6
RHOC antibody	>1 publications with NET1 and RHOC

Pathways associated with anti-NET1 antibody

Products by Pathway	Pathway Diagram
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies	Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
G Alpha (12/13) Signalling Events Pathway antibodies	G Alpha (12/13) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
NRAGE Signals Death Through JNK Pathway antibodies	NRAGE Signals Death Through JNK Pathway Diagram
Regulation Of RhoA Activity Pathway antibodies	Regulation Of RhoA Activity Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram
Signaling By Rho GTPases Pathway antibodies	Signaling By Rho GTPases Pathway Diagram
Signalling By NGF Pathway antibodies	Signalling By NGF Pathway Diagram

Diseases associated with anti-NET1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>16 publications with NET1 and Adenocarcinoma
Breast Neoplasms Antibodies	>14 publications with NET1 and Breast Neoplasms
Liver Diseases Antibodies	>11 publications with NET1 and Liver Diseases
Liver Neoplasms Antibodies	>10 publications with NET1 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>9 publications with NET1 and Carcinoma, Hepatocellular
Neoplasms, Experimental Antibodies	>3 publications with NET1 and Neoplasms, Experimental
Hemorrhage Antibodies	>2 publications with NET1 and Hemorrhage
Disease Models, Animal Antibodies	>2 publications with NET1 and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>2 publications with NET1 and Cell Transformation, Neoplastic
Cognition Disorders Antibodies	>1 publications with NET1 and Cognition Disorders

Organs/Tissues associated with anti-NET1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with NET1 and Brain
Liver Antibodies	>11 publications with NET1 and Liver
Vascular Antibodies	>7 publications with NET1 and Vascular
Blood Antibodies	>7 publications with NET1 and Blood
Nerve Antibodies	>6 publications with NET1 and Nerve
Stomach Antibodies	>5 publications with NET1 and Stomach
Embryonic Tissue Antibodies	>4 publications with NET1 and Embryonic Tissue
Skin Antibodies	>3 publications with NET1 and Skin
Eye Antibodies	>2 publications with NET1 and Eye
Muscle Antibodies	>2 publications with NET1 and Muscle