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IRF5 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421517

MBS421517 is an antibody targeting IRF5. Also known as Goat anti-IRF5 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IRF5, Polyclonal Antibody
Full Product Name
Goat anti-IRF5 Antibody
Product Gene Name
anti-IRF5 antibody
Product Synonym Names
IRF5; interferon regulatory factor 5; SLEB10; IRF5 antibody; interferon regulatory factor 5 antibody; SLEB10 antibody
Immunogen Sequence
QGPWPMHPAG MQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
IRF5 peptide (MBS426976) is used for blocking the activity of IRF5 antibody (MBS421517)
Molecular Weight
17,020 Da
OMIM
180300
Immunogen
Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1.
Epitope
C Terminus
Note
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IRF5 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-IRF5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer (data not shown).
Western Blot: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3ug/ml.
Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.

Western Blot (WB) of anti-IRF5 antibody

(0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IRF5 Polyclonal Antibody – Cat# MBS421517

Western Blot (WB) of anti-IRF5 antibody

HEK293 overexpressing human IRF5 and probed at 1ug/ml (mock transfection in lane B).
Goat Anti-IRF5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421517

Other Notes

Small volumes of anti-IRF5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IRF5. It may not necessarily be applicable to this product.

NCBI GI #
4504727
NCBI GeneID
3663
NCBI Accession #
NP_002191.1
NCBI Related Accession #
NP_116032.1; NP_001092097.1; NP_001092099.1
UniProt Secondary Accession #
Q1A7B4; Q64GA9; Q64GB1; Q64GB2; Q6RCM8; Q9BQF0; A4D1J8; A8DUA8; A8DUA9; E7EQ16; E7EW54
UniProt Related Accession #
Q13568
GenBank Nucleotide #
NM_002200.3
NCBI Official Synonym Full Names
interferon regulatory factor 5
NCBI Official Symbol
IRF5
NCBI Official Synonym Symbols
SLEB10
UniProt Protein Name
Interferon regulatory factor 5
Protein Family
Interferon regulatory factor
UniProt Gene Name
IRF5
UniProt Synonym Gene Names
IRF-5
UniProt Entry Name
IRF5_HUMAN
NCBI Protein Information
interferon regulatory factor 5

NCBI Summary for IRF5

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
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UniProt Comments for IRF5

IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: defense response to virus; positive regulation of apoptosis; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-12 production; positive regulation of transcription from RNA polymerase II promoter; response to muramyl dipeptide; response to peptidoglycan; transcription, DNA-dependent

Disease: Inflammatory Bowel Disease 14; Systemic Lupus Erythematosus, Susceptibility To, 10
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Research Articles on IRF5

1. four single nucleotide polymorphisms studied in IRF5 may affect neither Neuromyelitis optica nor Multiple sclerosis in the Southeastern Han Chinese population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRF5 antibody

 Reference Product  PubMed Publications
 IRF3 antibody  >47 publications with IRF5 and IRF3
 MYD88 antibody  >23 publications with IRF5 and MYD88
 TRAF6 antibody  >9 publications with IRF5 and TRAF6
 TBK1 antibody  >7 publications with IRF5 and TBK1
 STAT2 antibody  >6 publications with IRF5 and STAT2
 IRF6 antibody  >5 publications with IRF5 and IRF6
 RELA antibody  >3 publications with IRF5 and RELA
 TP53 antibody  >3 publications with IRF5 and TP53

Pathways associated with anti-IRF5 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Alpha/beta Signaling Pathway antibodies  Interferon Alpha/beta Signaling Pathway Diagram
 Interferon Gamma Signaling Pathway antibodies  Interferon Gamma Signaling Pathway Diagram
 Regulation Of Toll-like Receptor Signaling Pathway antibodies  Regulation Of Toll-like Receptor Signaling Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Toll-like Receptor Signaling Pathway antibodies  Toll-like Receptor Signaling Pathway Diagram

Diseases associated with anti-IRF5 antibody

 Disease Name  Pubmed Publications
 Lupus Erythematosus, Systemic Antibodies  >157 publications with IRF5 and Lupus Erythematosus, Systemic
 Inflammation Antibodies  >84 publications with IRF5 and Inflammation
 Arthritis, Rheumatoid Antibodies  >68 publications with IRF5 and Arthritis, Rheumatoid
 Necrosis Antibodies  >34 publications with IRF5 and Necrosis
 Scleroderma, Systemic Antibodies  >26 publications with IRF5 and Scleroderma, Systemic
 Cardiovascular Diseases Antibodies  >17 publications with IRF5 and Cardiovascular Diseases
 Fibrosis Antibodies  >15 publications with IRF5 and Fibrosis
 Lung Diseases Antibodies  >12 publications with IRF5 and Lung Diseases
 Liver Diseases Antibodies  >12 publications with IRF5 and Liver Diseases
 Colitis, Ulcerative Antibodies  >10 publications with IRF5 and Colitis, Ulcerative

Organs/Tissues associated with anti-IRF5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >126 publications with IRF5 and Blood
 Bone Antibodies  >16 publications with IRF5 and Bone
 Spleen Antibodies  >12 publications with IRF5 and Spleen
 Bone Marrow Antibodies  >12 publications with IRF5 and Bone Marrow
 Brain Antibodies  >12 publications with IRF5 and Brain
 Kidney Antibodies  >10 publications with IRF5 and Kidney
 Connective Tissue Antibodies  >10 publications with IRF5 and Connective Tissue
 Lung Antibodies  >8 publications with IRF5 and Lung
 Mouth Antibodies  >6 publications with IRF5 and Mouth
 Lymph Node Antibodies  >4 publications with IRF5 and Lymph Node
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