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CMG1/CCDC2/IFT74 (IFT74) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421519

MBS421519 is an antibody targeting IFT74. Also known as Goat anti-CMG1/CCDC2/IFT74 Antibody. Reactive in Tested: Human, Rat; Expected from sequence similarity: Human, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CMG1/CCDC2/IFT74, Polyclonal Antibody
Full Product Name
Goat anti-CMG1/CCDC2/IFT74 Antibody
Product Gene Name
Product Synonym Gene Name
CMG1; CCDC2
Product Synonym Names
IFT74; CCDC2; CMG-1; FLJ22621; MGC111562; intraflagellar transport 74 homolog (Chlamydomonas); capillary morphogenesis protein 1; coiled-coil domain containing 2; CMG1; IFT74 antibody; CCDC2 antibody; CMG-1 antibody; FLJ22621 antibody; MGC111562 antibody; intraflagellar transport 74 homolog (Chlamydomonas) antibody; capillary morphogenesis protein 1 antibody; coiled-coil domain containing 2 antibody; CMG1 antibody; CMG1 / CCDC2 / IFT74
Immunogen Sequence
KTIVDALHST SGN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Matching Pairs
Unconjugated Antibody: CMG1/CCDC2/IFT74 (MBS421519)
Biotin Conjugated Antibody: CMG1/CCDC2/IFT74 (MBS423701)
Antibody/Peptide Pairs
Molecular Weight
42,332 Da
OMIM
608040
Immunogen
Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IFT74 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-IFT74 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Momeni et al, BMC Neurol.2006 Dec 13;6: 44.; PMID: 17166276). Recommended concentration: 0.3-1ug/ml.
Immunofluorescence: Product was successfully used at 2.5mg/ml to stain rat cortical neurons (see picture and PMID: 17166276).

Western Blot (WB) of anti-IFT74 antibody

Staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-IFT74 Polyclonal Antibody – Cat# MBS421519

Immunohistochemistry (IHC) of anti-IFT74 antibody

(5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. )
Goat Anti-IFT74 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421519

Immunofluorescence (IF) of anti-IFT74 antibody

A) (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. )
Goat Anti-IFT74 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421519

Other Notes

Small volumes of anti-IFT74 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IFT74. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intraflagellar transport protein 74 homolog isoform a
NCBI Official Synonym Full Names
intraflagellar transport 74
NCBI Official Symbol
IFT74
NCBI Official Synonym Symbols
CMG1; CCDC2; CMG-1
UniProt Protein Name
Intraflagellar transport protein 74 homolog
UniProt Synonym Protein Names
Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2
UniProt Gene Name
IFT74
UniProt Synonym Gene Names
CCDC2; CMG1; CMG-1
UniProt Entry Name
IFT74_HUMAN
NCBI Protein Information
intraflagellar transport protein 74 homolog

UniProt Comments for IFT74

IFT74: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9p21.2

Cellular Component: centrosome; cilium; cytoplasmic membrane-bound vesicle; nucleus

Molecular Function: beta-tubulin binding; chromatin binding

Biological Process: cilium biogenesis; determination of left/right symmetry; heart development; negative regulation of epithelial cell proliferation; Notch signaling pathway; positive regulation of cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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