• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CRHR1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421554

MBS421554 is an antibody targeting CRHR1. Also known as Goat anti-CRHR1/CRF-R (aa 107 - 117) Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CRHR1/CRF-R, Polyclonal Antibody
Full Product Name
Goat anti-CRHR1/CRF-R (aa 107 - 117) Antibody
Product Gene Name
anti-CRHR1 antibody
Product Synonym Gene Name
CRF-R
Product Synonym Names
CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1,CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor
Immunogen Sequence
NEEKKSKVHY H
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
CRHR1 / CRF-R peptide (MBS426154) is used for blocking the activity of CRHR1/CRF-R antibody (MBS421554)
OMIM
122561
Immunogen
Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CRHR1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CRHR1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128,000
Western Blot: Approx 60kDa band observed in human brain (cerebellum), Colon, Rat Brain and Mouse spinal cord lysates(calculated MW of 47.7kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol.2001 Mar;59(3): 485-92; PMID: 11179443). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-CRHR1 antibody

(1ug/ml) staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CRHR1 Polyclonal Antibody – Cat# MBS421554

Immunofluorescence (IF) of anti-CRHR1 antibody

Confocal image (3um) of (0.3ug/ml) staining in green a dendrite of a neuronal cell in C57 mouse cortex. Counter-stained with DAPI (in blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.
Goat Anti-CRHR1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421554

Other Notes

Small volumes of anti-CRHR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CRHR1. It may not necessarily be applicable to this product.

NCBI GI #
19923245
NCBI GeneID
1394
NCBI Accession #
NP_004373.2
UniProt Secondary Accession #
Q13008; Q4QRJ1; Q9UK64; B4DIE9
UniProt Related Accession #
P34998
GenBank Nucleotide #
NM_004382.4
NCBI Official Full Name
corticotropin-releasing factor receptor 1 isoform 2
NCBI Official Synonym Full Names
corticotropin releasing hormone receptor 1
NCBI Official Symbol
CRHR1
NCBI Official Synonym Symbols
CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
UniProt Protein Name
Corticotropin-releasing factor receptor 1
UniProt Synonym Protein Names
Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1
Protein Family
Corticotropin-releasing factor receptor
UniProt Gene Name
CRHR1
UniProt Synonym Gene Names
CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
UniProt Entry Name
CRFR1_HUMAN
NCBI Protein Information
corticotropin-releasing factor receptor 1

NCBI Summary for CRHR1

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Readthrough transcription also exists between this gene and upstream GeneID:401884 (ADP-ribosylation factor 3 pseudogene), and the readthrough transcripts encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Dec 2014]
Show more

UniProt Comments for CRHR1

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: endosome; integral to membrane; integral to plasma membrane; intrinsic to plasma membrane; plasma membrane

Molecular Function: corticotrophin-releasing factor receptor activity; protein binding

Biological Process: adenylate cyclase activation; adrenocorticotropin hormone secretion; cell surface receptor linked signal transduction; female pregnancy; immune response; parturition
Show more

Research Articles on CRHR1

1. The results revealed that changes in depressive symptoms over time depended on both intervention group and genotypes (5-HTTLPR and CRHR1).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CRHR1 antibody

 Reference Product  PubMed Publications
 CRH antibody  >208 publications with CRHR1 and CRH
 POMC antibody  >38 publications with CRHR1 and POMC
 CRHBP antibody  >21 publications with CRHR1 and CRHBP
 UCN antibody  >21 publications with CRHR1 and UCN
 UCN2 antibody  >9 publications with CRHR1 and UCN2
 CREB1 antibody  >2 publications with CRHR1 and CREB1
 KCNMA1 antibody  >1 publications with CRHR1 and KCNMA1
 SLC2A1 antibody  >1 publications with CRHR1 and SLC2A1
 DLG1 antibody  >1 publications with CRHR1 and DLG1
 YY1 antibody  >1 publications with CRHR1 and YY1

Pathways associated with anti-CRHR1 antibody

 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram

Organs/Tissues associated with anti-CRHR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >180 publications with CRHR1 and Brain
 Embryonic Tissue Antibodies  >5 publications with CRHR1 and Embryonic Tissue
 Ovary Antibodies  >3 publications with CRHR1 and Ovary
 Eye Antibodies  >2 publications with CRHR1 and Eye
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000