• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

C2gnt-M (GCNT3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421572

MBS421572 is an antibody targeting GCNT3. Also known as Goat anti-C2GnT-M (aa 410 to 422) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
C2GnT-M (GCNT3), Polyclonal Antibody
Full Product Name
Goat anti-C2GnT-M (aa 410 to 422) Antibody
Product Gene Name
anti-GCNT3 antibody
Product Synonym Gene Name
C2GnT-M
Product Synonym Names
Goat Anti-C2GnT-M (aa 273 to 284) Antibody; GCNT3; glucosaminyl (N-acetyl) transferase 3, mucin type; C2/4GnT; C2GnT-M; C2GnT2; GnT-M; beta1,6-N-acetylglucosaminyltransferase; GCNT3 antibody; glucosaminyl (N-acetyl) transferase 3; mucin type antibody; C2/4GnT antibody; C2GnT-M antibody; C2GnT2 antibody; GnT-M antibody; beta1; 6-N-acetylglucosaminyltransferase antibody; C2GnT-M (aa 410 to 422)
Immunogen Sequence
NKFDPKVDDN ALQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
C2GnT-M peptide (MBS428443) is used for blocking the activity of C2GnT-M antibody (MBS421572)
Molecular Weight
50,864 Da
OMIM
606836
Immunogen
Peptide with sequence C-NKFDPKVDDNALQ, from the internal region (near C terminus) of the protein sequence according to NP_004742.1.
Epitope
Internal region (near C Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GCNT3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GCNT3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 55-60kDa band observed in Human Duodenum lysates (calculated MW of 50.9kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-GCNT3 antibody

(0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GCNT3 Polyclonal Antibody – Cat# MBS421572

Western Blot (WB) of anti-GCNT3 antibody

HEK293 overexpressing GCNT3 (RC202007) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-GCNT3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421572

Other Notes

Small volumes of anti-GCNT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GCNT3. It may not necessarily be applicable to this product.

NCBI GI #
4758422
NCBI GeneID
9245
NCBI Accession #
NP_004742.1
UniProt Related Accession #
O95395
GenBank Nucleotide #
NM_004751.2
NCBI Official Full Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
NCBI Official Synonym Full Names
glucosaminyl (N-acetyl) transferase 3, mucin type
NCBI Official Symbol
GCNT3
NCBI Official Synonym Symbols
GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
UniProt Protein Name
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
UniProt Synonym Protein Names
C2GnT-mucin type; C2GnT-M; hC2GnT-M; Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; C2/4GnT
Protein Family
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
UniProt Gene Name
GCNT3
UniProt Synonym Gene Names
C2GnT-M; hC2GnT-M; C2/4GnT
UniProt Entry Name
GCNT3_HUMAN
NCBI Protein Information
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

NCBI Summary for GCNT3

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]

UniProt Comments for GCNT3

GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.102; EC 2.4.1.150; Glycan Metabolism - O-glycan biosynthesis

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; integral to membrane; membrane

Molecular Function: acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: carbohydrate metabolic process; immunoglobulin production in mucosal tissue; intestinal absorption; O-glycan processing; protein amino acid O-linked glycosylation; tissue morphogenesis
Show more

Research Articles on GCNT3

1. This study provides the concepts toward the design of carbohydrate-dependent inhibition of EPEC and EHEC O157:H7 adhesion to human intestinal epithelial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GCNT3 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 Mucin Type O-Glycan Biosynthesis Pathway antibodies  Mucin Type O-Glycan Biosynthesis Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-glycan Biosynthesis, Mucin Type Core Pathway antibodies  O-glycan Biosynthesis, Mucin Type Core Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Mucin Core 1 And Core 2 O-glycosylation Pathway antibodies  Mucin Core 1 And Core 2 O-glycosylation Pathway Diagram

Diseases associated with anti-GCNT3 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >2 publications with GCNT3 and Immune System Diseases
 Hyperplasia Antibodies  >2 publications with GCNT3 and Hyperplasia
 Lung Diseases Antibodies  >1 publications with GCNT3 and Lung Diseases
 Neoplasm Metastasis Antibodies  >1 publications with GCNT3 and Neoplasm Metastasis
 Carcinoma Antibodies  >1 publications with GCNT3 and Carcinoma
 Prostatic Neoplasms Antibodies  >1 publications with GCNT3 and Prostatic Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with GCNT3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with GCNT3 and Liver Neoplasms
 Acute Kidney Injury Antibodies  >1 publications with GCNT3 and Acute Kidney Injury

Organs/Tissues associated with anti-GCNT3 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >3 publications with GCNT3 and Intestine
 Prostate Antibodies  >2 publications with GCNT3 and Prostate
 Blood Antibodies  >1 publications with GCNT3 and Blood
 Eye Antibodies  >1 publications with GCNT3 and Eye
 Kidney Antibodies  >1 publications with GCNT3 and Kidney
 Lung Antibodies  >1 publications with GCNT3 and Lung
 Muscle Antibodies  >1 publications with GCNT3 and Muscle
 Liver Antibodies  >1 publications with GCNT3 and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000