MARCH-I (MARCH1) Antibody – Goat Polyclonal

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

MARCH1: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Membrane protein, integral; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, multi-pass; Ligase

Chromosomal Location of Human Ortholog: 4q32.2

Cellular Component: cytoplasmic vesicle membrane; early endosome membrane; endoplasmic reticulum membrane; endosome; integral to membrane; late endosome membrane; lysosomal membrane; lysosome; plasma membrane; trans-Golgi network membrane

Molecular Function: ligase activity; MHC protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class II; immune response; protein polyubiquitination