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Urocortin 3 (UCN3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421585

MBS421585 is an antibody targeting UCN3. Also known as Goat anti-Urocortin 3 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Urocortin 3 (UCN3), Polyclonal Antibody
Full Product Name
Goat anti-Urocortin 3 Antibody
Product Gene Name
Product Synonym Names
UCN3; urocortin 3 (stresscopin); MGC119002; SCP; SPC; UCNIII; stresscopin; urocortin 3; urocortin III; UCN3 antibody; urocortin 3 (stresscopin) antibody; MGC119002 antibody; SCP antibody; SPC antibody; UCNIII antibody; stresscopin antibody; urocortin 3 antibody; urocortin III antibody; Urocortin 3; Urocortin 3
Immunogen Sequence
KPRQDTAKSP HRTK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
17,961 Da
OMIM
605901
Immunogen
Peptide with sequence C-KPRQDTAKSPHRTK, from the internal region of the protein sequence according to NP_444277.2.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-UCN3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-UCN3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: In transfected HEK293 transiently expressing full-length Human UCN3 (myc and DYKDDDDK tagged), a band of approx.19kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1- 2ug/ml.

Western Blot (WB) of anti-UCN3 antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DYKDDDDK tag probed (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Goat Anti-UCN3 Polyclonal Antibody – Cat# MBS421585

Other Notes

Small volumes of anti-UCN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UCN3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
urocortin-3 preproprotein
NCBI Official Synonym Full Names
urocortin 3
NCBI Official Symbol
UCN3
NCBI Official Synonym Symbols
SCP; SPC; UCNIII
UniProt Protein Name
Urocortin-3
UniProt Synonym Protein Names
Stresscopin; Urocortin III; Ucn III
Protein Family
UniProt Gene Name
UCN3
UniProt Synonym Gene Names
SPC; Ucn III
UniProt Entry Name
UCN3_HUMAN
NCBI Protein Information
urocortin-3

NCBI Summary for UCN3

This gene encodes a member of the sauvagine/corticotropin-releasing factor/urotensin I family of proteins. The encoded preproprotein is proteolytically processed to generate the mature peptide hormone, which is secreted by pancreatic beta and alpha cells. This hormone is an endogenous ligand for corticotropin-releasing factor receptor 2 and may regulate insulin secretion in response to plasma glucose levels. Patients with type 2 diabetes exhibit reduced levels of the encoded protein in beta cells. In the brain, the encoded protein may be responsible for the effects of stress on appetite. [provided by RefSeq, May 2016]
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UniProt Comments for UCN3

UCN3: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: extracellular space

Molecular Function: corticotropin-releasing hormone receptor 2 binding; G-protein-coupled receptor binding; hormone activity

Biological Process: cellular response to nutrient levels; digestion; G-protein signaling, adenylate cyclase activating pathway; response to stress
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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