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MSH4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421595

MBS421595 is an antibody targeting MSH4. Also known as Goat anti-MSH4 (internal) Antibody. Reactive in Expected from sequence similarity: Human, Mouse. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSH4, Polyclonal Antibody
Full Product Name
Goat anti-MSH4 (internal) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-MSH4 (C Terminus) Antibody; MSH4; mutS homolog 4 (E. coli); RP11-262K24.2; mutS homolog 4; MSH4 antibody; mutS homolog 4 (E. coli) antibody; RP11-262K24.2 antibody; mutS homolog 4 antibody; MSH4 (internal)
Immunogen Sequence
ETNSSTFMKE MKE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MSH4 peptide (MBS426193) is used for blocking the activity of MSH4 antibody (MBS421595)
Molecular Weight
104,756 Da
OMIM
602105
Immunogen
Peptide with sequence C-ETNSSTFMKEMKE, from the internal region of the protein sequence according to NP_002431.2.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MSH4 antibody
Peptide ELISA (EIA)
Application Notes for anti-MSH4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Preliminary experiments gave bands at approx 60kDa and 35kDa in Human Testis, Ovary and Uterus lysates and in Mouse and Rat Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 105kDa according to NP_002431.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Other Notes

Small volumes of anti-MSH4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mutS protein homolog 4
NCBI Official Synonym Full Names
mutS homolog 4
NCBI Official Symbol
MSH4
UniProt Protein Name
MutS protein homolog 4
UniProt Gene Name
MSH4
UniProt Synonym Gene Names
hMSH4
UniProt Entry Name
MSH4_HUMAN
NCBI Protein Information
mutS protein homolog 4

NCBI Summary for MSH4

This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011]
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UniProt Comments for MSH4

MSH4: Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis. Belongs to the DNA mismatch repair MutS family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: nucleus; synaptonemal complex

Molecular Function: ATP binding; damaged DNA binding; DNA binding; mismatched DNA binding; protein binding

Biological Process: female gamete generation; homologous chromosome segregation; meiotic mismatch repair; meiotic recombination; mismatch repair; ovarian follicle development; spermatogenesis; synapsis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-MSH4 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >17 publications with MSH4 and Testis
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