PAM Antibody – Goat Polyclonal

This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

PAM: Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 4.3.2.5; Vesicle; Lyase; EC 1.14.17.3

Chromosomal Location of Human Ortholog: 5q14-q21

Cellular Component: cell surface; extracellular space; integral to membrane; membrane; neuron projection; perikaryon; perinuclear region of cytoplasm; plasma membrane; secretory granule membrane; trans-Golgi network

Molecular Function: calcium ion binding; copper ion binding; L-ascorbic acid binding; peptidylamidoglycolate lyase activity; peptidylglycine monooxygenase activity; protein binding; protein kinase binding; zinc ion binding

Biological Process: central nervous system development; heart development; lactation; limb development; long-chain fatty acid metabolic process; maternal process involved in pregnancy; odontogenesis; ovulation cycle process; peptide amidation; protein amino acid amidation; protein homooligomerization; regulation of actin cytoskeleton organization and biogenesis; regulation of protein secretion; regulation of transcription from RNA polymerase II promoter; response to copper ion; response to drug; response to estradiol stimulus; response to glucocorticoid stimulus; response to hypoxia; response to pH; toxin metabolic process