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NOVA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421610

MBS421610 is an antibody targeting NOVA1. Also known as Goat anti-NOVA1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NOVA1, Polyclonal Antibody
Full Product Name
Goat anti-NOVA1 Antibody
Product Gene Name
Product Synonym Names
NOVA1; neuro-oncological ventral antigen 1; Nova-1; paraneoplastic Ri antigen; ventral neuron-specific protein 1; NOVA1 antibody; neuro-oncological ventral antigen 1 antibody; Nova-1 antibody; paraneoplastic Ri antigen antibody; ventral neuron-specific protein 1 antibody
Immunogen Sequence
REMPQNVAKT EPVS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NOVA1 peptide (MBS425688) is used for blocking the activity of NOVA1 antibody (MBS421610)
Molecular Weight
49,253 Da
OMIM
602157
Immunogen
Peptide with sequence C-REMPQNVAKTEPVS, from the internal region of the protein sequence according to NP_002506.2 ; NP_006480.2 ; NP_006482.1.
Epitope
Internal region
Note
This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NOVA1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NOVA1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of selected nuclei. Recommended concentration, 2-4ug/ml.
Western Blot: In transfected HEK293 transiently expressing NOVA1 a band of approx.52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml.

Western Blot (WB) of anti-NOVA1 antibody

(0. 01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NOVA1 Polyclonal Antibody – Cat# MBS421610

Western Blot (WB) of anti-NOVA1 antibody

HEK293 overexpressing NOVA1 (RC210407) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-NOVA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421610

Immunohistochemistry (IHC) of anti-NOVA1 antibody

(2.5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-NOVA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421610

Other Notes

Small volumes of anti-NOVA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NOVA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding protein Nova-1 isoform 1
NCBI Official Synonym Full Names
NOVA alternative splicing regulator 1
NCBI Official Symbol
NOVA1
NCBI Official Synonym Symbols
Nova-1
UniProt Protein Name
RNA-binding protein Nova-1
UniProt Synonym Protein Names
Neuro-oncological ventral antigen 1; Onconeural ventral antigen 1; Paraneoplastic Ri antigen; Ventral neuron-specific protein 1
Protein Family
UniProt Gene Name
NOVA1
UniProt Entry Name
NOVA1_HUMAN
NCBI Protein Information
RNA-binding protein Nova-1

NCBI Summary for NOVA1

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for NOVA1

NOVA1: May regulate RNA splicing or metabolism in a specific subset of developing neurons. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 14q

Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleus

Molecular Function: mRNA binding; RNA binding

Biological Process: locomotory behavior; nuclear mRNA splicing, via spliceosome; regulation of mRNA processing; RNA processing; RNA splicing; spinal cord development; synaptic transmission
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NOVA1 antibody

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