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PNPLA3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421616

MBS421616 is an antibody targeting PNPLA3. Also known as Goat anti-PNPLA3/Adiponutrin Antibody. Reactive in Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PNPLA3/Adiponutrin, Polyclonal Antibody
Full Product Name
Goat anti-PNPLA3/Adiponutrin Antibody
Product Gene Name
anti-PNPLA3 antibody
Product Synonym Names
Pnpla3; patatin-like phospholipase domain containing 3; Adpn; adiponutrin; Pnpla3 antibody; patatin-like phospholipase domain containing 3 antibody; Adpn antibody; adiponutrin antibody; Adiponutrin; PNPLA3 / Adiponutrin
Immunogen Sequence
EHDICPKVKS TN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PNPLA3 / Adiponutrin peptide (MBS426868) is used for blocking the activity of PNPLA3/Adiponutrin antibody (MBS421616)
Immunogen
Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
Note
This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PNPLA3 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PNPLA3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Skin shows membranous staining of basak kayer cells.
Recommended concentration, 4-6ug/ml.
Western Blot: Approx 45kDa band observed in Mouse Adrenal gland lysates
(calculated MW of 42.6kDa according to Mouse NP_473429.2)
Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room tempeture.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 8ug/mL.

Western Blot (WB) of anti-PNPLA3 antibody

(0. 05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PNPLA3 Polyclonal Antibody – Cat# MBS421616

Western Blot (WB) of anti-PNPLA3 antibody

(0. 01ug/ml) staining of Mouse (A) and Rat (B) Adrenal gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PNPLA3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421616

Immunohistochemistry (IHC) of anti-PNPLA3 antibody

(3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-PNPLA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421616

Immunohistochemistry (IHC) of anti-PNPLA3 antibody

(8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-PNPLA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421616

Immunohistochemistry (IHC) of anti-PNPLA3 antibody

Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Goat Anti-PNPLA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421616

Immunofluorescence (IF) of anti-PNPLA3 antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-PNPLA3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS421616

Other Notes

Small volumes of anti-PNPLA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PNPLA3. It may not necessarily be applicable to this product.

NCBI GI #
144226245
NCBI GeneID
116939
NCBI Accession #
NP_473429.2
UniProt Related Accession #
Q91WW7
GenBank Nucleotide #
NM_054088.3
NCBI Official Full Name
patatin-like phospholipase domain-containing protein 3
NCBI Official Synonym Full Names
patatin-like phospholipase domain containing 3
NCBI Official Symbol
Pnpla3
NCBI Official Synonym Symbols
Adpn
UniProt Protein Name
Patatin-like phospholipase domain-containing protein 3
UniProt Synonym Protein Names
Acylglycerol O-acyltransferase (EC:2.3.1.-); Adiponutrin; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon
Protein Family
Patatin-like phospholipase domain-containing protein
UniProt Gene Name
Pnpla3
UniProt Synonym Gene Names
Adpn; iPLA2-epsilon
UniProt Entry Name
PLPL3_MOUSE
NCBI Protein Information
patatin-like phospholipase domain-containing protein 3

UniProt Comments for PNPLA3

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Amino Acid Metabolism - tyrosine; Transferase; Membrane protein, integral; Amino Acid Metabolism - phenylalanine; EC 3.1.1.3; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; Phospholipase

Cellular Component: integral to membrane; membrane

Molecular Function: 3-hydroxybutyryl-CoA thiolase activity; 3-ketopimelyl-CoA thiolase activity; acetyltransferase activity; acyl-CoA N-acyltransferase activity; acylglycerol O-acyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; benzoyl acetate-CoA thiolase activity; C-acyltransferase activity; C-palmitoyltransferase activity; carnitine O-acyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; dihydrolipoamide S-acyltransferase activity; diolein transacylation activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; hydrolase activity; keto acid formate lyase activity; L-2-aminoadipate N-acetyltransferase activity; malonyltransferase activity; mono-olein transacylation activity; myristoyltransferase activity; N-acetyltransferase activity; N-acyltransferase activity; N-palmitoyltransferase activity; N-succinyltransferase activity; O-acetyltransferase activity; O-acyltransferase activity; O-octanoyltransferase activity; O-palmitoyltransferase activity; O-sinapoyltransferase activity; O-succinyltransferase activity; octanoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; palmitoyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; phospholipase A2 activity; protein-cysteine S-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; Ras palmitoyltransferase activity; S-acetyltransferase activity; S-acyltransferase activity; S-malonyltransferase activity; S-succinyltransferase activity; serine O-acyltransferase activity; sinapoyltransferase activity; sterol O-acyltransferase activity; succinyltransferase activity; transferase activity; transferase activity, transferring acyl groups; triacylglycerol lipase activity

Biological Process: lipid catabolic process; lipid metabolic process; metabolic process; triacylglycerol biosynthetic process; triacylglycerol catabolic process
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Research Articles on PNPLA3

1. Neither ablation nor overexpression of wild-type PNPLA3 affects liver fat content in mice, whereas hepatic overexpression of the human 148M transgene causes steatosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PNPLA3 antibody

 Reference Product  PubMed Publications
 Lpl antibody  >1 publications with PNPLA3 and Lpl
 Lpin1 antibody  >1 publications with PNPLA3 and Lpin1
 Dgat2 antibody  >1 publications with PNPLA3 and Dgat2

Pathways associated with anti-PNPLA3 antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodeling Of DAG And TAG Pathway antibodies  Acyl Chain Remodeling Of DAG And TAG Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipids/Glycerophospholipids Metabolism Pathway antibodies  Glycerolipids/Glycerophospholipids Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-PNPLA3 antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >295 publications with PNPLA3 and Fatty Liver
 Liver Diseases Antibodies  >285 publications with PNPLA3 and Liver Diseases
 Non-alcoholic Fatty Liver Disease Antibodies  >184 publications with PNPLA3 and Non-alcoholic Fatty Liver Disease
 Fibrosis Antibodies  >116 publications with PNPLA3 and Fibrosis
 Liver Diseases, Alcoholic Antibodies  >71 publications with PNPLA3 and Liver Diseases, Alcoholic
 Liver Cirrhosis, Alcoholic Antibodies  >67 publications with PNPLA3 and Liver Cirrhosis, Alcoholic
 Carcinoma, Hepatocellular Antibodies  >62 publications with PNPLA3 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >42 publications with PNPLA3 and Inflammation
 Liver Neoplasms Antibodies  >32 publications with PNPLA3 and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >14 publications with PNPLA3 and Cardiovascular Diseases

Organs/Tissues associated with anti-PNPLA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Joint Antibodies  >8 publications with PNPLA3 and Joint
 Brain Antibodies  >2 publications with PNPLA3 and Brain
 Eye Antibodies  >1 publications with PNPLA3 and Eye
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