Goat Anti-CCT3 Polyclonal Antibody – Cat# MBS421657

(0. 01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CCT3 Antibody – Goat Polyclonal

Name: CCT3 Antibody; Polyclonal; Goat; Mouse/Rat/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421657
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421657

MBS421657 is an antibody targeting CCT3. Also known as Goat anti-CCT3/TCP1 Antibody. Reactive in Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCT3/TCP1, Polyclonal Antibody

Full Product Name

Goat anti-CCT3/TCP1 Antibody

Product Gene Name

anti-CCT3 antibody

Product Synonym Gene Name

TCP1

Product Synonym Names

CCT3; chaperonin containing TCP1, subunit 3 (gamma); CCT-gamma; CCTG; PIG48; TCP-1-gamma; TRIC5; OTTHUMP00000025735; T-complex protein 1, gamma subunit; TCP1 (t-complex-1) ring complex, polypeptide 5; chaperonin containing TCP1, subunit 3; CCT3 antibody; chaperonin containing TCP1; subunit 3 (gamma) antibody; CCT-gamma antibody; CCTG antibody; PIG48 antibody; TCP-1-gamma antibody; TRIC5 antibody; OTTHUMP00000025735 antibody; T-complex protein 1; gamma subunit antibody; TCP1 (t-complex-1) ring complex; polypeptide 5 antibody; chaperonin containing TCP1; subunit 3 antibody; CCT3 / TCP1

Immunogen Sequence

GHKKKGDDQS RQGG

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow, Dog

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

CCT3 / TCP1 peptide (MBS425218) is used for blocking the activity of CCT3/TCP1 antibody (MBS421657)

Molecular Weight

56,431 Da

OMIM

600114

Immunogen

Peptide with sequence C-GHKKKGDDQSRQGG, from the C-Terminus (near) of the protein sequence according to NP_005989.3; NP_001008883.1; NP_001008800.1.

Epitope

C-Terminus (near)

Note

This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CCT3 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-CCT3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 65kDa band observed in Mouse and Rat Testis lysates (calculated MW of 60.5kDa according to Human NP_005989.3 and 60.6kDa according to Mouse NP_033966.1 and Rat NP_954522.1). Recommended concentration: 0.01-0.03ug/ml.

Other Notes

Small volumes of anti-CCT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCT3. It may not necessarily be applicable to this product.

NCBI GI #

63162572

NCBI GeneID

7203

NCBI Accession #

NP_005989.3

NCBI Related Accession #

NP_001008883.1; NP_001008800.1

UniProt Secondary Accession #

Q5SZY1; Q9BR64; A6NE14

UniProt Related Accession #

P49368

GenBank Nucleotide #

NM_005998.4

NCBI Official Full Name

T-complex protein 1 subunit gamma isoform a

NCBI Official Synonym Full Names

chaperonin containing TCP1 subunit 3

NCBI Official Symbol

CCT3

NCBI Official Synonym Symbols

CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma

UniProt Protein Name

T-complex protein 1 subunit gamma

UniProt Synonym Protein Names

CCT-gamma; hTRiC5

Protein Family

Thermosome

UniProt Gene Name

CCT3

UniProt Synonym Gene Names

CCTG; TRIC5; TCP-1-gamma

UniProt Entry Name

TCPG_HUMAN

NCBI Protein Information

T-complex protein 1 subunit gamma

NCBI Summary for CCT3

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]

UniProt Comments for CCT3

CCT3: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: chaperonin-containing T-complex; cytoplasm; cytoskeleton; cytosol; microtubule; myelin sheath; plasma membrane

Molecular Function: ATP binding; protein binding; unfolded protein binding

Biological Process: binding of sperm to zona pellucida; pore complex biogenesis; positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization

Research Articles on CCT3

1. Overexpression of CCT3 in the nuclei of cancerous cells is associated with hepatocellular carcinoma progression. CCT3 may be a target that affects the activation of STAT3 in HCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CCT3 antibody

Products by Pathway	Pathway Diagram
Assembly Of The Primary Cilium Pathway antibodies	Assembly Of The Primary Cilium Pathway Diagram
Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies	Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
BBSome-mediated Cargo-targeting To Cilium Pathway antibodies	BBSome-mediated Cargo-targeting To Cilium Pathway Diagram
Cargo Trafficking To The Periciliary Membrane Pathway antibodies	Cargo Trafficking To The Periciliary Membrane Pathway Diagram
Chaperonin-mediated Protein Folding Pathway antibodies	Chaperonin-mediated Protein Folding Pathway Diagram
Cooperation Of PDCL (PhLP1) And TRiC/CCT In G-protein Beta Folding Pathway antibodies	Cooperation Of PDCL (PhLP1) And TRiC/CCT In G-protein Beta Folding Pathway Diagram
Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies	Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
Folding Of Actin By CCT/TriC Pathway antibodies	Folding Of Actin By CCT/TriC Pathway Diagram
Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies	Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-CCT3 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>8 publications with CCT3 and Carcinoma
Carcinoma, Hepatocellular Antibodies	>6 publications with CCT3 and Carcinoma, Hepatocellular
Adenocarcinoma Antibodies	>5 publications with CCT3 and Adenocarcinoma
Liver Neoplasms Antibodies	>5 publications with CCT3 and Liver Neoplasms
Liver Diseases Antibodies	>4 publications with CCT3 and Liver Diseases
Neoplasm Metastasis Antibodies	>1 publications with CCT3 and Neoplasm Metastasis
Kidney Diseases Antibodies	>1 publications with CCT3 and Kidney Diseases
Adenoma, Liver Cell Antibodies	>1 publications with CCT3 and Adenoma, Liver Cell
Neoplasms, Experimental Antibodies	>1 publications with CCT3 and Neoplasms, Experimental
Abnormalities, Drug-Induced Antibodies	>1 publications with CCT3 and Abnormalities, Drug-Induced

Organs/Tissues associated with anti-CCT3 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>5 publications with CCT3 and Liver
Blood Antibodies	>3 publications with CCT3 and Blood
Kidney Antibodies	>2 publications with CCT3 and Kidney
Brain Antibodies	>2 publications with CCT3 and Brain
Bone Antibodies	>1 publications with CCT3 and Bone
Muscle Antibodies	>1 publications with CCT3 and Muscle
Ovary Antibodies	>1 publications with CCT3 and Ovary
Eye Antibodies	>1 publications with CCT3 and Eye
Lung Antibodies	>1 publications with CCT3 and Lung
Testis Antibodies	>1 publications with CCT3 and Testis