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Proenkephalin (PENK) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421669

MBS421669 is an antibody targeting PENK. Also known as Goat anti-Proenkephalin (mouse) Antibody. Reactive in Tested: Mouse, RatExpected from sequence similarity: Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Proenkephalin (PENK), Polyclonal Antibody
Full Product Name
Goat anti-Proenkephalin (mouse) Antibody
Product Gene Name
Product Synonym Names
proenkephalin; proenkephalin antibody; Proenkephalin; Proenkephalin (mouse)
Immunogen Sequence
YKDSSKQDES H
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Immunogen
Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PENK antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-PENK antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.05ug/ml.
Western Blot: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry (IHC) of anti-PENK antibody

(0. 02ug/ml) staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.
Goat Anti-PENK Polyclonal Antibody – Cat# MBS421669

Immunohistochemistry (IHC) of anti-PENK antibody

(0. 02ug/ml) staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med. , Budapest, Hungary.
Goat Anti-PENK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421669

Other Notes

Small volumes of anti-PENK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PENK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
proenkephalin-A
NCBI Official Synonym Full Names
preproenkephalin
NCBI Official Symbol
Penk
NCBI Official Synonym Symbols
ENK; PPA; Penk1; AI326464
UniProt Protein Name
Proenkephalin-A
UniProt Synonym Protein Names
SynenkephalinMet-enkephalinAlternative name(s):Opioid growth factor; OGF
Protein Family
UniProt Gene Name
Penk
UniProt Synonym Gene Names
Penk1; OGF
UniProt Entry Name
PENK_MOUSE
NCBI Protein Information
proenkephalin-A

UniProt Comments for PENK

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted; Secreted, signal peptide; Hormone
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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