Goat Anti-ADCYAP1 Polyclonal Antibody – Cat# MBS421693

(1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ADCYAP1 Antibody – Goat Polyclonal

Name: ADCYAP1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421693
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421693

MBS421693 is an antibody targeting ADCYAP1. Also known as Goat anti-PACAP Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PACAP (ADCYAP1), Polyclonal Antibody

Full Product Name

Goat anti-PACAP Antibody

Product Gene Name

anti-ADCYAP1 antibody

Product Synonym Gene Name

PACAP

Product Synonym Names

ADCYAP1; adenylate cyclase activating polypeptide 1 (pituitary); MGC126852; PACAP; OTTHUMP00000162201; adenylate cyclase activating polypeptide; ADCYAP1 antibody; adenylate cyclase activating polypeptide 1 (pituitary) antibody; MGC126852 antibody; PACAP antibody; OTTHUMP00000162201 antibody; adenylate cyclase activating polypeptide antibody

Immunogen Sequence

GKRYKQRVKN KGRR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

PACAP peptide (MBS426079) is used for blocking the activity of PACAP antibody (MBS421693)

Molecular Weight

18,835 Da

OMIM

102980

Immunogen

Peptide with sequence C-GKRYKQRVKNKGRR, from the C Terminus of the protein sequence according to NP_001108.2.

Epitope

C Terminus

Note

Reported variants represent identical protein: NP_001093203.1, NP_001108.2.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-ADCYAP1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-ADCYAP1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 25kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 18.8kDa according to NP_001108.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-ADCYAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADCYAP1. It may not necessarily be applicable to this product.

NCBI GI #

153266795

NCBI GeneID

116

NCBI Accession #

NP_001108.2

UniProt Secondary Accession #

Q52LQ0; B2R7N4

UniProt Related Accession #

P18509

GenBank Nucleotide #

NM_001117.4

NCBI Official Full Name

pituitary adenylate cyclase-activating polypeptide preproprotein

NCBI Official Synonym Full Names

adenylate cyclase activating polypeptide 1

NCBI Official Symbol

ADCYAP1

NCBI Official Synonym Symbols

PACAP

UniProt Protein Name

Pituitary adenylate cyclase-activating polypeptide

UniProt Synonym Protein Names

PACAP-related peptideAlternative name(s):PRP-48

Protein Family

Glucagon family neuropeptides

UniProt Gene Name

ADCYAP1

UniProt Synonym Gene Names

PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

UniProt Entry Name

PACA_HUMAN

NCBI Protein Information

pituitary adenylate cyclase-activating polypeptide

NCBI Summary for ADCYAP1

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

UniProt Comments for ADCYAP1

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18p11

Cellular Component: extracellular region

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; pituitary adenylate cyclase activating polypeptide activity; protein binding; receptor binding

Biological Process: adenylate cyclase activation; cAMP-mediated signaling; cell-cell signaling; female pregnancy; neurite development; neuropeptide signaling pathway; positive regulation of GTPase activity; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein localization

Research Articles on ADCYAP1

1. PACAP is involved in the pathogenesis of migraine rather than tension-type headache

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ADCYAP1 antibody

Products by Pathway	Pathway Diagram
Activation Of TRKA Receptors Pathway antibodies	Activation Of TRKA Receptors Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Glucagon-type Ligand Receptors Pathway antibodies	Glucagon-type Ligand Receptors Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
NGF Signalling Via TRKA From The Plasma Membrane Pathway antibodies	NGF Signalling Via TRKA From The Plasma Membrane Pathway Diagram
NGF-independant TRKA Activation Pathway antibodies	NGF-independant TRKA Activation Pathway Diagram
Renin Secretion Pathway antibodies	Renin Secretion Pathway Diagram

Diseases associated with anti-ADCYAP1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>129 publications with ADCYAP1 and Nervous System Diseases
Brain Diseases Antibodies	>76 publications with ADCYAP1 and Brain Diseases
Inflammation Antibodies	>58 publications with ADCYAP1 and Inflammation
Disease Models, Animal Antibodies	>42 publications with ADCYAP1 and Disease Models, Animal
Necrosis Antibodies	>35 publications with ADCYAP1 and Necrosis
Pain Antibodies	>22 publications with ADCYAP1 and Pain
Brain Injuries Antibodies	>12 publications with ADCYAP1 and Brain Injuries
Hyperglycemia Antibodies	>10 publications with ADCYAP1 and Hyperglycemia
Hyperplasia Antibodies	>10 publications with ADCYAP1 and Hyperplasia
Lung Neoplasms Antibodies	>9 publications with ADCYAP1 and Lung Neoplasms

Organs/Tissues associated with anti-ADCYAP1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>595 publications with ADCYAP1 and Brain
Nerve Antibodies	>307 publications with ADCYAP1 and Nerve
Blood Antibodies	>218 publications with ADCYAP1 and Blood
Pancreas Antibodies	>88 publications with ADCYAP1 and Pancreas
Adrenal Gland Antibodies	>62 publications with ADCYAP1 and Adrenal Gland
Testis Antibodies	>47 publications with ADCYAP1 and Testis
Lung Antibodies	>46 publications with ADCYAP1 and Lung
Skin Antibodies	>14 publications with ADCYAP1 and Skin
Uterus Antibodies	>4 publications with ADCYAP1 and Uterus