Goat Anti-MARCH10 Polyclonal Antibody – Cat# MBS421725

(0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MARCH10 Antibody – Goat Polyclonal

Name: MARCH10 Antibody; Polyclonal; Goat; Rat/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421725
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421725

MBS421725 is an antibody targeting MARCH10. Also known as Goat anti-MARCH10 Antibody. Reactive in Tested: Rat; Expected from sequence similarity: Human, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MARCH10, Polyclonal Antibody

Full Product Name

Goat anti-MARCH10 Antibody

Product Gene Name

anti-MARCH10 antibody

Product Synonym Names

MARCH10; membrane-associated ring finger (C3HC4) 10; FLJ35757; MARCH-X; RNF190; ring finger protein 190; MARCH10 antibody; membrane-associated ring finger (C3HC4) 10 antibody; FLJ35757 antibody; MARCH-X antibody; RNF190 antibody; ring finger protein 190 antibody

Immunogen Sequence

SRNYPQPRTE E

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Rat; Expected from sequence similarity: Human, Rat, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

MARCH10 peptide (MBS425084) is used for blocking the activity of MARCH10 antibody (MBS421725)

Molecular Weight

90,511 Da

OMIM

613337

Immunogen

Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2.

Epitope

Internal region

Note

This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-MARCH10 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-MARCH10 antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx.90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.

Other Notes

Small volumes of anti-MARCH10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCH10. It may not necessarily be applicable to this product.

NCBI GI #

154937342

NCBI GeneID

162333

NCBI Accession #

NP_001094345.1

NCBI Related Accession #

NP_689811.2

UniProt Secondary Accession #

Q8IYS7; Q8N7Z7; D3DU09

UniProt Related Accession #

Q8NA82

GenBank Nucleotide #

NM_001100875.2

NCBI Official Full Name

probable E3 ubiquitin-protein ligase MARCH10 isoform 1

NCBI Official Synonym Full Names

membrane associated ring-CH-type finger 10

NCBI Official Symbol

MARCH10

NCBI Official Synonym Symbols

RNF190; MARCH-X

UniProt Protein Name

Probable E3 ubiquitin-protein ligase MARCH10

UniProt Synonym Protein Names

Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; MARCH-X; RING finger protein 190

Protein Family

Probable E3 ubiquitin-protein ligase

UniProt Gene Name

MARCH10

UniProt Synonym Gene Names

RNF190; MARCH-X

UniProt Entry Name

MARHA_HUMAN

NCBI Protein Information

probable E3 ubiquitin-protein ligase MARCH10

NCBI Summary for MARCH10

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

UniProt Comments for MARCH10

MARCH10: E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 17q23.2

Molecular Function: ligase activity; protein binding; zinc ion binding

Biological Process: protein ubiquitination

Research Articles on MARCH10

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-MARCH10 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with MARCH10 and Testis