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Stromal Antigen 2/STAG2 (STAG2) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421897

MBS421897 is an antibody targeting STAG2. Also known as Goat anti-Stromal antigen 2/STAG2 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Stromal antigen 2/STAG2, Polyclonal Antibody
Full Product Name
Goat anti-Stromal antigen 2/STAG2 Antibody
Product Gene Name
anti-STAG2 antibody
Product Synonym Names
Goat Anti-STAG2 Antibody; STAG2; stromal antigen 2; DKFZp686P168; DKFZp781H1753; FLJ25871; SA-2; SA2; bA517O1.1; OTTHUMP00000024338; OTTHUMP00000043514; OTTHUMP00000043515; OTTHUMP00000081754; OTTHUMP00000081755; SCC3 homolog 2; STAG2 antibody; stromal antigen 2 antibody; DKFZp686P168 antibody; DKFZp781H1753 antibody; FLJ25871 antibody; SA-2 antibody; SA2 antibody; bA517O1.1 antibody; OTTHUMP00000024338 antibody; OTTHUMP00000043514 antibody; OTTHUMP00000043515 antibody; OTTHUMP00000081754 antibody; OTTHUMP00000081755 antibody; SCC3 homolog 2 antibody; Stromal antigen 2; Stromal antigen 2 / STAG2
Immunogen Sequence
SRGSTVRSKK SKPST
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Stromal antigen 2 / STAG2 peptide (MBS425372) is used for blocking the activity of Stromal antigen 2/STAG2 antibody (MBS421897)
Molecular Weight
145,751 Da
OMIM
300826
Immunogen
Peptide with sequence C-SRGSTVRSKKSKPST, from the internal region of the protein sequence according to NP_001036214.1; NP_006594.3.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_001036214.1; NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-STAG2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-STAG2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 160kDa band observed in lysates of cell line K562 and ~140kDa in rodent Spleen while ~150kDa in Mouse Thymus lysates (calculated MW of 146kDa according to Human NP_001036214.1 and 140kDa according to Mouse NP_001071180.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-STAG2 antibody

(0. 03ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-STAG2 Polyclonal Antibody – Cat# MBS421897

Western Blot (WB) of anti-STAG2 antibody

(0.3ug/ml) staining of Mouse Spleen (A), Mouse Thymus (B) and Rat Spleen (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-STAG2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421897

Other Notes

Small volumes of anti-STAG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STAG2. It may not necessarily be applicable to this product.

NCBI GI #
112789526
NCBI GeneID
10735
NCBI Accession #
NP_001036214.1
NCBI Related Accession #
NP_006594.3
UniProt Secondary Accession #
O00540; Q5JTI6; Q68DE9; Q9H1N8; B1AMT5; D3DTF5
UniProt Related Accession #
Q8N3U4
GenBank Nucleotide #
NM_001042749.2
NCBI Official Full Name
cohesin subunit SA-2 isoform a
NCBI Official Synonym Full Names
stromal antigen 2
NCBI Official Symbol
STAG2
NCBI Official Synonym Symbols
SA2; SA-2; SCC3B; bA517O1.1
UniProt Protein Name
Cohesin subunit SA-2
UniProt Synonym Protein Names
SCC3 homolog 2; Stromal antigen 2
Protein Family
Cofilin
UniProt Gene Name
STAG2
UniProt Synonym Gene Names
SA2
UniProt Entry Name
STAG2_HUMAN
NCBI Protein Information
cohesin subunit SA-2

NCBI Summary for STAG2

The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
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UniProt Comments for STAG2

STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: actin cytoskeleton; chromatin; chromosome; chromosome, pericentric region; cytosol; intermediate filament cytoskeleton; membrane; nucleolus; nucleoplasm; nucleus; plasma membrane

Molecular Function: protein binding

Biological Process: cell division; meiotic cell cycle; mitosis; negative regulation of DNA endoreduplication; protein sumoylation; sister chromatid cohesion
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Research Articles on STAG2

1. Characterization of C-terminal nuclear localization signal of the human SA2 stromalin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-STAG2 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram

Diseases associated with anti-STAG2 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >11 publications with STAG2 and Leukemia, Myeloid
 Urinary Bladder Neoplasms Antibodies  >7 publications with STAG2 and Urinary Bladder Neoplasms
 Carcinoma, Transitional Cell Antibodies  >4 publications with STAG2 and Carcinoma, Transitional Cell
 Cell Transformation, Neoplastic Antibodies  >3 publications with STAG2 and Cell Transformation, Neoplastic
 Abnormalities, Multiple Antibodies  >3 publications with STAG2 and Abnormalities, Multiple
 Anemia Antibodies  >2 publications with STAG2 and Anemia
 Adenocarcinoma Antibodies  >2 publications with STAG2 and Adenocarcinoma
 Lung Neoplasms Antibodies  >2 publications with STAG2 and Lung Neoplasms
 Learning Disorders Antibodies  >2 publications with STAG2 and Learning Disorders
 Congenital Abnormalities Antibodies  >2 publications with STAG2 and Congenital Abnormalities

Organs/Tissues associated with anti-STAG2 antibody

 Organ/Tissue Name  Pubmed Publications
 Bladder Antibodies  >8 publications with STAG2 and Bladder
 Blood Antibodies  >6 publications with STAG2 and Blood
 Bone Antibodies  >4 publications with STAG2 and Bone
 Muscle Antibodies  >2 publications with STAG2 and Muscle
 Stomach Antibodies  >2 publications with STAG2 and Stomach
 Lung Antibodies  >2 publications with STAG2 and Lung
 Brain Antibodies  >2 publications with STAG2 and Brain
 Prostate Antibodies  >1 publications with STAG2 and Prostate
 Skin Antibodies  >1 publications with STAG2 and Skin
 Testis Antibodies  >1 publications with STAG2 and Testis
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