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BTLA Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421917

MBS421917 is an antibody targeting BTLA. Also known as Goat anti-BTLA Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BTLA, Polyclonal Antibody
Full Product Name
Goat anti-BTLA Antibody
Product Gene Name
Product Synonym Names
BTLA; B and T lymphocyte associated; BTLA1; CD272; FLJ16065; MGC129743; B and T lymphocyte attenuator; BTLA antibody; B and T lymphocyte associated antibody; BTLA1 antibody; CD272 antibody; FLJ16065 antibody; MGC129743 antibody; B and T lymphocyte attenuator antibody
Immunogen Sequence
KEAPTEYASI CVRS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
BTLA peptide (MBS426864) is used for blocking the activity of BTLA antibody (MBS421917)
Molecular Weight
27,317 Da
OMIM
607925
Immunogen
Peptide with sequence KEAPTEYASICVRS, from the C Terminus of the protein sequence according to NP_861445.3; NP_001078826.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-BTLA antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-BTLA antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx.35kDa band observed in Mouse Spleen lysates (calculated MW of 32.8kDa according to Human NP_861445.3 and of 34.2kDa according to Mouse NP_001032808.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-BTLA antibody

(1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-BTLA Polyclonal Antibody – Cat# MBS421917

Other Notes

Small volumes of anti-BTLA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BTLA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
B- and T-lymphocyte attenuator isoform 1
NCBI Official Synonym Full Names
B and T lymphocyte associated
NCBI Official Symbol
BTLA
NCBI Official Synonym Symbols
BTLA1; CD272
UniProt Protein Name
B- and T-lymphocyte attenuator
UniProt Synonym Protein Names
B- and T-lymphocyte-associated protein; CD_antigen: CD272
UniProt Gene Name
BTLA
UniProt Entry Name
BTLA_HUMAN
NCBI Protein Information
B- and T-lymphocyte attenuator

NCBI Summary for BTLA

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
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UniProt Comments for BTLA

BTLA: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: integral to membrane; plasma membrane

Biological Process: adaptive immune response; T cell costimulation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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