Goat Anti-USP16 Polyclonal Antibody – Cat# MBS421934

USP16 Antibody – Goat Polyclonal

Name: USP16 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421934
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421934

MBS421934 is an antibody targeting USP16. Also known as Goat anti-USP16 Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Horse, Dog, Cow. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

USP16, Polyclonal Antibody

Full Product Name

Goat anti-USP16 Antibody

Product Gene Name

anti-USP16 antibody

Product Synonym Names

Goat Anti-USP16 / UBP-M Antibody; USP16; ubiquitin specific peptidase 16; UBP-M; deubiquitinating enzyme 16; ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific protease 16; ubiquitin thiolesterase 16; ubiquitin-specific processing protease 16; USP16 antibody; ubiquitin specific peptidase 16 antibody; UBP-M antibody; deubiquitinating enzyme 16 antibody; ubiquitin carboxyl-terminal hydrolase 16 antibody; ubiquitin specific protease 16 antibody; ubiquitin thiolesterase 16 antibody; ubiquitin-specific processing protease 16 antibody

Immunogen Sequence

NVEWNICQDC KTDNK

Clonality

Polyclonal

Host

Goat

Species Reactivity

Expected from sequence similarity: Human, Mouse, Rat, Horse, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

USP16 peptide (MBS426680) is used for blocking the activity of USP16 antibody (MBS421934)

Molecular Weight

46,599 Da

OMIM

604735

Immunogen

Peptide with sequence NVEWNICQDCKTDNK, from the internal region of the protein sequence according to NP_001027582.1; NP_001001992.1.

Epitope

Internal region

Note

Reported variants represent identical protein: NP_001027582.1 NP_006438.1

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-USP16 antibody

Peptide ELISA (EIA)

Application Notes for anti-USP16 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands).

Other Notes

Small volumes of anti-USP16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP16. It may not necessarily be applicable to this product.

NCBI GI #

75992941

NCBI GeneID

10600

NCBI Accession #

NP_001027582.1

NCBI Related Accession #

NP_001001992.1

UniProt Secondary Accession #

Q53GP7; Q53HA0; Q5VKN8; Q8NEL3; Q9H3E6; A8MU43; B3KN13; B4DFV8; B4DY37; D3DSD9

UniProt Related Accession #

Q9Y5T5

GenBank Nucleotide #

NM_001032410.1

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase 16 isoform a

NCBI Official Synonym Full Names

ubiquitin specific peptidase 16

NCBI Official Symbol

USP16

NCBI Official Synonym Symbols

UBPM; UBP-M

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase 16

UniProt Synonym Protein Names

Deubiquitinating enzyme 16

UniProt Gene Name

USP16

UniProt Entry Name

UBP16_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase 16

NCBI Summary for USP16

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for USP16

USP16: a ubiquitous ubiquitin-processing protease that may de-ubiquitinate one or more critical proteins that are involved in the condensation of mitotic chromosomes, possibly acting selectively on histones H2A and H2B, the major ubiquitinated proteins of chromatin. Is phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity; histone binding; transcription coactivator activity; ubiquitin binding; ubiquitin-specific protease activity; zinc ion binding

Biological Process: cell cycle; cell division; histone deubiquitination; mitosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translational elongation; protein homotetramerization; regulation of cell cycle; regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process

Research Articles on USP16

1. The data unveil a unique mechanism by which Usp16 promotes the localization and maintenance of Plk1 on the kinetochores for proper chromosome alignment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP16 antibody

Reference Product	PubMed Publications
HERC2 antibody	>1 publications with USP16 and HERC2

Pathways associated with anti-USP16 antibody

Products by Pathway	Pathway Diagram
Integrated Breast Cancer Pathway antibodies	Integrated Breast Cancer Pathway Diagram

Diseases associated with anti-USP16 antibody

Disease Name	Pubmed Publications
Neurobehavioral Manifestations Antibodies	>3 publications with USP16 and Neurobehavioral Manifestations
Body Weight Changes Antibodies	>1 publications with USP16 and Body Weight Changes
Liver Diseases Antibodies	>1 publications with USP16 and Liver Diseases
Fatty Liver Antibodies	>1 publications with USP16 and Fatty Liver
Anemia Antibodies	>1 publications with USP16 and Anemia
Diabetes Mellitus Antibodies	>1 publications with USP16 and Diabetes Mellitus
Lung Neoplasms Antibodies	>1 publications with USP16 and Lung Neoplasms
Necrosis Antibodies	>1 publications with USP16 and Necrosis

Organs/Tissues associated with anti-USP16 antibody

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>2 publications with USP16 and Bone Marrow
Blood Antibodies	>2 publications with USP16 and Blood
Lymph Node Antibodies	>1 publications with USP16 and Lymph Node
Thyroid Antibodies	>1 publications with USP16 and Thyroid
Lymph Antibodies	>1 publications with USP16 and Lymph
Lung Antibodies	>1 publications with USP16 and Lung
Muscle Antibodies	>1 publications with USP16 and Muscle
Mammary Gland Antibodies	>1 publications with USP16 and Mammary Gland
Liver Antibodies	>1 publications with USP16 and Liver