Goat Anti-SIGLEC8 Polyclonal Antibody – Cat# MBS421954

(0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SIGLEC8 Antibody – Goat Polyclonal

Name: SIGLEC8 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421954
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421954

MBS421954 is an antibody targeting SIGLEC8. Also known as Goat anti-SIGLEC8 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SIGLEC8, Polyclonal Antibody

Full Product Name

Goat anti-SIGLEC8 Antibody

Product Gene Name

anti-SIGLEC8 antibody

Product Synonym Names

SIGLEC8; sialic acid binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC-8; SIGLEC8L; SIGLEC8 antibody; sialic acid binding Ig-like lectin 8 antibody; MGC59785 antibody; SAF2 antibody; SIGLEC-8 antibody; SIGLEC8L antibody

Immunogen Sequence

SYKSQLNYKT KQ

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

SIGLEC8 peptide (MBS426267) is used for blocking the activity of SIGLEC8 antibody (MBS421954)

Molecular Weight

46,434 Da

OMIM

605639

Immunogen

Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence according to NP_055257.2.

Epitope

Internal region

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-SIGLEC8 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-SIGLEC8 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54. 0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood.2003 Jun 15;101(12): 5014-20.; PMID: 12609831). Recommended concentration: 0.3-1ug/ml.

Other Notes

Small volumes of anti-SIGLEC8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIGLEC8. It may not necessarily be applicable to this product.

NCBI GI #

94538343

NCBI GeneID

27181

NCBI Accession #

NP_055257.2

UniProt Secondary Accession #

Q7Z728

UniProt Related Accession #

Q9NYZ4

GenBank Nucleotide #

NM_014442.2

NCBI Official Full Name

sialic acid-binding Ig-like lectin 8

NCBI Official Synonym Full Names

sialic acid binding Ig like lectin 8

NCBI Official Symbol

SIGLEC8

NCBI Official Synonym Symbols

SAF2; SIGLEC-8; SIGLEC8L

UniProt Protein Name

Sialic acid-binding Ig-like lectin 8

UniProt Synonym Protein Names

Sialoadhesin family member 2; SAF-2

Protein Family

Sialic acid-binding Ig-like lectin

UniProt Gene Name

SIGLEC8

UniProt Synonym Gene Names

SAF2; Siglec-8; SAF-2

UniProt Entry Name

SIGL8_HUMAN

NCBI Protein Information

sialic acid-binding Ig-like lectin 8

NCBI Summary for SIGLEC8

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

UniProt Comments for SIGLEC8

SIGLEC8: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.33-q13.41

Cellular Component: integral to membrane

Molecular Function: carbohydrate binding; protein binding; transmembrane receptor activity

Biological Process: cell adhesion; signal transduction

Research Articles on SIGLEC8

1. Inflammation results in up-regulation of immuneinhibitory Siglec-8 and Siglec-9 sialoglycan ligands on human airways.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SIGLEC8 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Organs/Tissues associated with anti-SIGLEC8 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>26 publications with SIGLEC8 and Blood
Spleen Antibodies	>1 publications with SIGLEC8 and Spleen
Connective Tissue Antibodies	>1 publications with SIGLEC8 and Connective Tissue
Muscle Antibodies	>1 publications with SIGLEC8 and Muscle