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GCKR Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422063

MBS422063 is an antibody targeting GCKR. Also known as Goat anti-GCKR Antibody. Reactive in Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GCKR, Polyclonal Antibody
Full Product Name
Goat anti-GCKR Antibody
Product Gene Name
Product Synonym Names
GCKR; glucokinase (hexokinase 4) regulator; GKRP; glucokinase (hexokinase 4) regulatory protein; glucokinase regulator; glucokinase regulatory protein; hexokinase 4; GCKR antibody; glucokinase (hexokinase 4) regulator antibody; GKRP antibody; glucokinase (hexokinase 4) regulatory protein antibody; glucokinase regulator antibody; glucokinase regulatory protein antibody; hexokinase 4 antibody
Immunogen Sequence
KRFQHVIETP EPGK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GCKR peptide (MBS426333) is used for blocking the activity of GCKR antibody (MBS422063)
Molecular Weight
16,477 Da
OMIM
600842
Immunogen
Peptide with sequence KRFQHVIETPEPGK -C, from the N Terminus of the protein sequence according to NP_001477.1.
Epitope
N Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GCKR antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GCKR antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 70kDa band observed in Mouse and Rat Liver lysates (calculated MW of 68.7kDa according to Human NP_001477.1 and of 68.9kDa according to Rat NP_037252.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-GCKR antibody

(0.1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GCKR Polyclonal Antibody – Cat# MBS422063

Other Notes

Small volumes of anti-GCKR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GCKR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glucokinase regulatory protein
NCBI Official Synonym Full Names
glucokinase (hexokinase 4) regulator
NCBI Official Symbol
GCKR
NCBI Official Synonym Symbols
GKRP; FGQTL5
UniProt Protein Name
Glucokinase regulatory protein
UniProt Gene Name
GCKR
UniProt Synonym Gene Names
GKRP; Glucokinase regulator
UniProt Entry Name
GCKR_HUMAN
NCBI Protein Information
glucokinase regulatory protein

NCBI Summary for GCKR

This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008]
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UniProt Comments for GCKR

GCKR: a key regulator of glucokinase in the liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. When expressed alone, both glucokinase and GCKR localize predominantly to the cytoplasm. Co-expression induces both proteins to localize in the nucleus.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: carbohydrate binding; protein binding

Biological Process: carbohydrate metabolic process; negative regulation of glucokinase activity; protein import into nucleus, translocation; response to fructose stimulus; urate metabolic process

Disease: Fasting Plasma Glucose Level Quantitative Trait Locus 5
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GCKR antibody

 Reference Product  PubMed Publications
 GCK antibody  >45 publications with GCKR and GCK
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