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LIG1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422092

MBS422092 is an antibody targeting LIG1. Also known as Goat anti-LIG1/DNA ligase I Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LIG1/DNA ligase I, Polyclonal Antibody
Full Product Name
Goat anti-LIG1/DNA ligase I Antibody
Product Gene Name
anti-LIG1 antibody
Product Synonym Names
LIG1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; DNA ligase I; LIG1 antibody; ligase I; DNA; ATP-dependent antibody; MGC117397 antibody; MGC130025 antibody; DNA ligase I antibody; DNA ligase I; LIG1 / DNA ligase I
Immunogen Sequence
RVREDKQPEQ ATTS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
LIG1 / DNA ligase I peptide (MBS426505) is used for blocking the activity of LIG1/DNA ligase I antibody (MBS422092)
Molecular Weight
98,228 Da
OMIM
126391
Immunogen
Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.
Epitope
Internal region (near C-Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-LIG1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-LIG1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-LIG1 antibody

(0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-LIG1 Polyclonal Antibody – Cat# MBS422092

Other Notes

Small volumes of anti-LIG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LIG1. It may not necessarily be applicable to this product.

NCBI GI #
4557719
NCBI GeneID
3978
NCBI Accession #
NP_000225.1
UniProt Secondary Accession #
Q2TB12; Q32P23; B2RAI8; B4DTU4
UniProt Related Accession #
P18858
GenBank Nucleotide #
NM_000234.2
NCBI Official Full Name
DNA ligase 1 isoform 1
NCBI Official Synonym Full Names
DNA ligase 1
NCBI Official Symbol
LIG1
UniProt Protein Name
DNA ligase 1
UniProt Synonym Protein Names
DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
Protein Family
DNA ligase
UniProt Gene Name
LIG1
UniProt Entry Name
DNLI1_HUMAN
NCBI Protein Information
DNA ligase 1

NCBI Summary for LIG1

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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UniProt Comments for LIG1

LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Protein type: Ligase; EC 6.5.1.1; DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2-q13.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; nucleoplasm; nucleus

Molecular Function: ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; metal ion binding

Biological Process: anatomical structure morphogenesis; base-excision repair; cell division; DNA ligation; DNA ligation during DNA repair; DNA metabolic process; DNA repair; mismatch repair; nucleotide-excision repair, DNA gap filling; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair; V(D)J recombination
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Research Articles on LIG1

1. Data suggest that DNA ligase I (LigI)-deficient 46BR.1G1 cells represent a model to investigate the biological effects of sub-lethal levels of DNA insults.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIG1 antibody

 Reference Product  PubMed Publications
 PCNA antibody  >46 publications with LIG1 and PCNA
 FEN1 antibody  >31 publications with LIG1 and FEN1
 APEX1 antibody  >16 publications with LIG1 and APEX1
 CDK2 antibody  >2 publications with LIG1 and CDK2
 POLD3 antibody  >1 publications with LIG1 and POLD3
 POLD1 antibody  >1 publications with LIG1 and POLD1
 CHEK1 antibody  >1 publications with LIG1 and CHEK1

Pathways associated with anti-LIG1 antibody

 Products by Pathway  Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-LIG1 antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >10 publications with LIG1 and Lung Diseases
 Lung Neoplasms Antibodies  >10 publications with LIG1 and Lung Neoplasms
 Heart Diseases Antibodies  >2 publications with LIG1 and Heart Diseases
 Cognition Disorders Antibodies  >2 publications with LIG1 and Cognition Disorders
 Breast Neoplasms Antibodies  >2 publications with LIG1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >2 publications with LIG1 and Cardiovascular Diseases
 Carcinoma, Squamous Cell Antibodies  >2 publications with LIG1 and Carcinoma, Squamous Cell
 Infertility, Female Antibodies  >1 publications with LIG1 and Infertility, Female
 Anemia Antibodies  >1 publications with LIG1 and Anemia
 Neoplasms, Experimental Antibodies  >1 publications with LIG1 and Neoplasms, Experimental

Organs/Tissues associated with anti-LIG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >15 publications with LIG1 and Lung
 Brain Antibodies  >9 publications with LIG1 and Brain
 Blood Antibodies  >7 publications with LIG1 and Blood
 Skin Antibodies  >3 publications with LIG1 and Skin
 Liver Antibodies  >3 publications with LIG1 and Liver
 Prostate Antibodies  >2 publications with LIG1 and Prostate
 Stomach Antibodies  >2 publications with LIG1 and Stomach
 Nerve Antibodies  >2 publications with LIG1 and Nerve
 Kidney Antibodies  >2 publications with LIG1 and Kidney
 Heart Antibodies  >2 publications with LIG1 and Heart
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