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PPP4C Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422199

MBS422199 is an antibody targeting PPP4C. Also known as Goat anti-PPP4C Antibody. Reactive in Tested: Human, Rat; Expected from sequence similarity: Human, Rat, Dog, Rabbit. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPP4C, Polyclonal Antibody
Full Product Name
Goat anti-PPP4C Antibody
Product Gene Name
Product Synonym Names
PPP4C; protein phosphatase 4 (formerly X), catalytic subunit; PP4; PPH3; PPX; Protein phosphatase 4, catalytic subunit; PPP4C antibody; protein phosphatase 4 (formerly X); catalytic subunit antibody; PP4 antibody; PPH3 antibody; PPX antibody; Protein phosphatase 4; catalytic subunit antibody
Immunogen Sequence
PQETRGIPSK KP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Rat, Dog, Rabbit
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PPP4C peptide (MBS426128) is used for blocking the activity of PPP4C antibody (MBS422199)
Molecular Weight
35,080 Da
OMIM
602035
Immunogen
Peptide with sequence C-PQETRGIPSKKP, from the C Terminus of the protein sequence according to NP_002711.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PPP4C antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PPP4C antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 35kDa band observed in HEK293, A431 and HeLa lysates and in Rat Spleen and Lung lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-PPP4C antibody

(0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PPP4C Polyclonal Antibody – Cat# MBS422199

Western Blot (WB) of anti-PPP4C antibody

(0.3ug/ml) staining of Rat Spleen (A) and Lung (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PPP4C Polyclonal Antibody, Western Blot (WB) – Cat# MBS422199

Immunohistochemistry (IHC) of anti-PPP4C antibody

(2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-PPP4C Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS422199

Other Notes

Small volumes of anti-PPP4C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP4C. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 4 catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP-X; PP4C; PPH3; PPP4
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Synonym Protein Names
Protein phosphatase X; PP-X
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
UniProt Entry Name
PP4C_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit

UniProt Comments for PPP4C

PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasm; microtubule organizing center; nucleoplasm; nucleus; plasma membrane

Molecular Function: lamin binding; metal ion binding; NF-kappaB-inducing kinase activity; protein binding; protein complex binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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