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PDE1A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422282

MBS422282 is an antibody targeting PDE1A. Also known as Goat anti-PDE1A Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDE1A, Polyclonal Antibody
Full Product Name
Goat anti-PDE1A Antibody
Product Gene Name
Product Synonym Names
PDE1A; phosphodiesterase 1A, calmodulin-dependent; HCAM1; HSPDE1A; MGC26303; 3',5' cyclic nucleotide phosphodiesterase; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; calmodulin-dependent phosphodiesterase; phosphodiesterase-1A; 3'; 5' cyclic nucleotide phosphodiesterase antibody; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase antibody; calmodulin-dependent phosphodiesterase antibody; HCAM1 antibody; HSPDE1A antibody; MGC26303 antibody; phosphodiesterase 1A; calmodulin-dependent antibody; phosphodiesterase-1A antibody; PDE1A antibody
Immunogen Sequence
DLHKNSEDLV NAE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PDE1A peptide (MBS427193) is used for blocking the activity of PDE1A antibody (MBS422282)
Molecular Weight
59,271 Da
OMIM
171890
Immunogen
Peptide with sequence C-DLHKNSEDLVNAE, from the C Terminus of the protein sequence according to NP_005010.2.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_005010.2) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PDE1A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PDE1A antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 62.3kDa according to NP_005010.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-PDE1A antibody

(1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PDE1A Polyclonal Antibody – Cat# MBS422282

Other Notes

Small volumes of anti-PDE1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 1A
NCBI Official Symbol
PDE1A
NCBI Official Synonym Symbols
HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
UniProt Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
UniProt Synonym Protein Names
61 kDa Cam-PDE; hCam-1
UniProt Gene Name
PDE1A
UniProt Synonym Gene Names
Cam-PDE 1A
UniProt Entry Name
PDE1A_HUMAN
NCBI Protein Information
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

NCBI Summary for PDE1A

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
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UniProt Comments for PDE1A

PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Phosphodiesterase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin binding; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; metal ion binding

Biological Process: metabolic process; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDE1A antibody

 Reference Product  PubMed Publications
 ADCY1 antibody  >1 publications with PDE1A and ADCY1
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